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581001 Cyclic AMP EIA Kit
Adenosine 3',5'-cyclic monophosphate EIA Kit; cAMP EIA Kit; Adenosine 3',5'-cyclic mononucleotide EIA Kit; Cyclic AMP ELISA Kit
Cyclic AMP EIA Kit Workbook Links
Cayman offers a variety of analysis tools to assist you.
Chemical structure definitions are available for many Cayman products. See Chemical Structure Database for details.
You may be eligible to receive a free sample Cyclic AMP EIA Kit under the Cayman Challenge program.
Let Cayman analyze your samples for you. See EIA Service for details and availability.
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Limit of detection:
80% B/B0: 3 pmol/ml
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Sensitivity:
50% B/B0: 20 pmol/ml
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Adenosine 3',5' cyclic mononucleotide (cAMP) is a ubiquitous cellular second messenger that is a critical component of a signal transduction pathway linking membrane receptors and their ligands to the activation of internal cellular enzymatic activity and gene expression. cAMP is synthesized from ATP by membrane-bound adenylate cyclase. Binding of certain ligands or hormones to their specific G-protein couple receptors activates GTP binding proteins (Gs or Gi) which either stimulate or inhibit adenylate cyclase. cAMP activates or inhibits various enzymes or cascade of enzymes by promoting their phosphorylation or dephosphorylation. The cAMP signal is neutralized by hydrolysis of cAMP to AMP by phosphodiesterases. Therefore, the concentration of cAMP in a cell is a function of the ratio of the rate of synthesis from ATP by adenylate cyclase and its rate of breakdown to AMP by specific phosphodiesterases. The Cayman Chemical cAMP assay is a competitive EIA that exhibits a limit of quantification of 0.1 pmol/ml (at 80% B/B0) for acetylated cAMP. Non-acetylated cAMP can be assayed with an IC50 of approximately 20 pmol/ml and a detection limit of 3 pmol/ml.
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Purchase 581001 Cyclic AMP EIA Kit
Pricing is for North America only. Other customers should contact a distributor in their region.
This product is also available to buy in bulk quantities.
Please contact our
Sales Department
for a quote or to purchase.
Cayman strives to be a reliable biochemical reagent vendor by providing the best possible products and services.
To ask for assistance with one of our products please contact our Technical Help staff.
Warning This product is not for human or veterinary use.
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Pricing updated 2010-03-16.
Prices are subject to change without notice.
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Many of our kits require a source of UltraPure Water. If you do not have a source it is available for purchase from Cayman Chemical. Please check the Additional Items Required section of this kit booklet to verify if Ultra Pure Water is needed.
Please note that the 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution. Please see the individual kit booklets for these details or contact techserv@caymanchem.com.
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