Limit of detection: 80% B/B₀: 3 pmol/ml · Sensitivity: 50% B/B₀: 20 pmol/ml · Adenosine 3',5' cyclic mononucleotide (cAMP) is a ubiquitous cellular second messenger that is a critical component of a signal transduction pathway linking membrane receptors and their ligands to the activation of internal cellular enzymatic activity and gene expression. cAMP is synthesized from ATP by membrane-bound adenylate cyclase. Binding of certain ligands or hormones to their specific G-protein couple receptors activates GTP binding proteins (Gs or Gi) which either stimulate or inhibit adenylate cyclase. cAMP activates or inhibits various enzymes or cascade of enzymes by promoting their phosphorylation or dephosphorylation. The cAMP signal is neutralized by hydrolysis of cAMP to AMP by phosphodiesterases. Therefore, the concentration of cAMP in a cell is a function of the ratio of the rate of synthesis from ATP by adenylate cyclase and its rate of breakdown to AMP by specific phosphodiesterases. The Cayman Chemical cAMP assay is a competitive EIA that exhibits a limit of quantification of 0.1 pmol/ml (at 80% B/B₀) for acetylated cAMP as well as an IC₅₀ value of 0.5 pmol/ml. Non-acetylated cAMP can be assayed with an IC₅₀ value of approximately 20 pmol/ml and a detection limit of 3 pmol/ml.

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