Cyclic AMP EIA Kit (without Acetic Anhydride)
Cayman Chemical Item Number 581002
cAMP EIA Kit (without Acetic Anhydride); Adenosine 3',5'-cyclic monophosphate EIA Kit (without Acetic Anhydride); Adenosine 3',5'-cyclic mononucleotide EIA Kit (without Acetic Anhydride); Cyclic AMP ELISA Kit (without Acetic Anhydride)
Cyclic AMP EIA Kit (without Acetic Anhydride)
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Description
cAMP is a ubiquitous cellular second messenger that is a critical component of a signal transduction pathway linking membrane receptors and their ligands to the activation of internal cellular enzymatic activity and gene expression. cAMP is synthesized from ATP by membrane-bound adenylate cyclase and activates or inhibits various enzymes or cascade of enzymes by promoting their phosphorylation or dephosphorylation. Cayman’s cAMP assay is a competitive EIA that permits cAMP measurements within the standard curve range of 0.08-10 pmol/ml, typically with a limit of quantification of 0.1 pmol/ml (80% B/B0) for acetylated cAMP. Non-acetylated cAMP can be assayed with an IC50 value (50% B/B0) of approximately 20 pmol/ml and a detection limit (80% B/B0) of 3 pmol/ml.
| Synonyms |
- cAMP EIA Kit (without Acetic Anhydride)
- Adenosine 3',5'-cyclic monophosphate EIA Kit (without Acetic Anhydride)
- Adenosine 3',5'-cyclic mononucleotide EIA Kit (without Acetic Anhydride)
- Cyclic AMP ELISA Kit (without Acetic Anhydride)
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| Stability |
1 year |
| Storage |
-20°C |
| Shipping |
Wet ice
in continental US; may vary elsewhere
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| Specificity |
| Acetylated cAMP |
100% |
| cAMP |
100% |
| cGMP |
1.5% |
| Acetylated cGMP |
0.69% |
| Acetylated Adenosine |
<0.01% |
| Acetylated AMP |
<0.01% |
| Adenosine |
<0.01% |
| AMP |
<0.01% |
| ATP |
<0.01% |
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- All 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution.
- Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay.
Pricing updated 2012-02-12.
Prices are subject to change without notice.
To ask for assistance with one of our products please contact a Technical Support Scientist.
Warning This product is not for human or veterinary use.
You may be eligible to receive a free sample Cyclic AMP EIA Kit (without Acetic Anhydride) under the Cayman Challenge program.
Let Cayman analyze your samples for you. See EIA Service for details and availability.
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Batch-specific Information
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Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.
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