Guanosine 3',5'-cyclic mononucleotide EIA Kit; Cyclic GMP ELISA Kit; cGMP EIA Kit; Guanosine 3',5'-cyclic monophosphate EIA Kit

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Chemical structure definitions are available for many Cayman products. See Chemical Structure Database for details.

You may be eligible to receive a free sample Cyclic GMP EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

Limit of detection: 80% B/B0: 1 pmol/ml · Sensitivity: 50% B/B0: 5.2 pmol/ml · The Cayman Chemical cGMP Assay is a competitive EIA that can be used for quantification of cGMP directly obtained from cell lysates, tissue homogenates, plasma or urine. The EIA typically displays an IC50 (50% B/B0) of approximately 6 pmol/ml and a detection limit (80% B/B0) of less than 2 pmol/ml. Since the antibody used in this assay was prepared against a cGMP-carrier protein conjugate, antibody binding is increased if an acetyl group is present on the 2' hydroxyl group of the cGMP. The optional acetylation procedure for both samples and standards increases the sensitivity of the assay approximately 10 fold. A protocol for acetylating both the standards and samples prior to performing the assay is provided. Basal levels of cGMP in cell lysates can often be measured without acetylation, but results will depend on the type and number of cells being utilized. Platelets produce approximately 1.5-2.5 pmol cGMP/109 platelets under basal conditions.1,2,3 Cells such as NG108-15 cells and monocytes produce considerably more cGMP than platelets (approximately 0.1-1 pmol/106 cells).4,5

1  Chen, L., Salafranca, M.N., Mehta, J.L. Cyclooxygenase inhibition decreases nitric oxide synthase activity in human platelets. Am J Physiol 42 H1854-H1859 (1997). 2  Miller, O.V., Gorman, R.R. Modulation of platelet cyclic nucleotide content by PGE1 and the prostaglandin endoperoxide PGG2. J Cyclic Nucleotide Res 2 79-87 (1976). 3  Wu, C., Ko, F., Kuo, S., et al. YC-1 inhibited human platelet aggregation through NO-independent activation of soluble guanylate cyclase. Br J Pharmacol 116 1973-1978 (1995). 4  Arima, T., Ohshima, Y., Mizuno, T., et al. Cyclic GMP elevation by 5-hydroxytryptamine is due to nitric oxide derived from endogenous nitrosothiol in NG108-15 cells. Biochem Biophys Res Commun 227 473-478 (1996). 5  Kolb, J.P., Paul-Eugene, N., Damais, C., et al. Interleukin-4 stimulates cGMP production by IFN-γ-activated human monocytes. Involvement of the nitric oxide synthase pathway. J Biol Chem 269 9811-9816 (1994).

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Many of our kits require a source of UltraPure Water. If you do not have a source it is available for purchase from Cayman Chemical. Please check the Additional Items Required section of this kit booklet to verify if Ultra Pure Water is needed. Please note that the 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution. Please see the individual kit booklets for these details or contact techserv@caymanchem.com.