Cyclic GMP EIA Kit (without Acetic Anhydride)

Cayman Chemical Item Number 581022

Cyclic GMP ELISA Kit (without Acetic Anhydride); Guanosine 3',5'-cyclic monophosphate EIA Kit (without Acetic Anhydride); Guanosine 3',5'-cyclic mononucleotide EIA Kit (without Acetic Anhydride); cGMP EIA Kit (without Acetic Anhydride)

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Cyclic GMP EIA Kit (without Acetic Anhydride) Analysis Tools

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Description

cGMP is a key intracellular second messenger molecule which transduces cellular signaling events in response to a variety of hormones, autacoids and drugs. cGMP is synthesized from GTP by both membrane-bound and soluble guanlyate cyclase enzymes. The relative abundance of cGMP within a given cell can serve as a marker for activation by agonists acting through particulate guanylate cyclase at the cell surface, or intracellular activation of soluble guanylate cyclase by nitric oxide. Cayman’s cGMP Assay is a competitive EIA that can be used for quantification of cGMP directly obtained from cell lysates, tissue homogenates, plasma or urine. The EIA typically displays an IC₅₀ value (50% B/B₀) of approximately 5 pmol/ml and a detection limit (80% B/B₀) of approximately 1 pmol/ml. Since the antibody used in this assay was prepared against a cGMP-carrier protein conjugate, antibody binding is increased if an acetyl group is present on the 2’ hydroxyl group of the cGMP. The optional acetylation procedure for both samples and standards increases the sensitivity of the assay approximately ten-fold.

Synonyms

Cyclic GMP ELISA Kit (without Acetic Anhydride)
Guanosine 3',5'-cyclic monophosphate EIA Kit (without Acetic Anhydride)
Guanosine 3',5'-cyclic mononucleotide EIA Kit (without Acetic Anhydride)
cGMP EIA Kit (without Acetic Anhydride)

Stability

1 year

Storage

-20°C

Shipping

Wet ice in continental US; may vary elsewhere

Specificity

Acetylated cGMP	100%
cGMP	9%
Dibutyryl cGMP	0.8%
Acetylated cAMP	0.05%
Acetylated adenosine	<0.01%
Acetylated AMP	<0.01%
Acetylated cytidine	<0.01%
Acetylated guanosine	<0.01%
Acetylated uridine	<0.01%
AMP	<0.01%
ATP	<0.01%
cAMP	<0.01%
8-bromo-cGMP	<0.01%

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581022-480SOLID

Cyclic GMP EIA Kit (without Acetic Anhydride) Analysis Tools

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