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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

Cyclic GMP EIA Kit (without Acetic Anhydride)

Cayman Chemical Item Number 581022

Cyclic GMP ELISA Kit (without Acetic Anhydride); Guanosine 3',5'-cyclic monophosphate EIA Kit (without Acetic Anhydride); Guanosine 3',5'-cyclic mononucleotide EIA Kit (without Acetic Anhydride); cGMP EIA Kit (without Acetic Anhydride)

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Cyclic GMP EIA Kit (without Acetic Anhydride) Analysis Tools

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Description

cGMP is a key intracellular second messenger molecule which transduces cellular signaling events in response to a variety of hormones, autacoids and drugs. cGMP is synthesized from GTP by both membrane-bound and soluble guanlyate cyclase enzymes. The relative abundance of cGMP within a given cell can serve as a marker for activation by agonists acting through particulate guanylate cyclase at the cell surface, or intracellular activation of soluble guanylate cyclase by nitric oxide. Cayman’s cGMP Assay is a competitive EIA that can be used for quantification of cGMP directly obtained from cell lysates, tissue homogenates, plasma or urine. The EIA typically displays an IC50 value (50% B/B0) of approximately 5 pmol/ml and a detection limit (80% B/B0) of approximately 1 pmol/ml. Since the antibody used in this assay was prepared against a cGMP-carrier protein conjugate, antibody binding is increased if an acetyl group is present on the 2’ hydroxyl group of the cGMP. The optional acetylation procedure for both samples and standards increases the sensitivity of the assay approximately ten-fold.
Synonyms
  • Cyclic GMP ELISA Kit (without Acetic Anhydride)
  • Guanosine 3',5'-cyclic monophosphate EIA Kit (without Acetic Anhydride)
  • Guanosine 3',5'-cyclic mononucleotide EIA Kit (without Acetic Anhydride)
  • cGMP EIA Kit (without Acetic Anhydride)
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
Acetylated cGMP 100%
cGMP 9%
Dibutyryl cGMP 0.8%
Acetylated cAMP 0.05%
Acetylated adenosine <0.01%
Acetylated AMP <0.01%
Acetylated cytidine <0.01%
Acetylated guanosine <0.01%
Acetylated uridine <0.01%
AMP <0.01%
ATP <0.01%
cAMP <0.01%
8-bromo-cGMP <0.01%
Show all 13 Hide all but first 3
581022-480SOLID
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Solid Plate 5 ea
Potassium Hydroxide 1 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
Cyclic GMP AChE Tracer 500 dtn
Cyclic GMP EIA Antiserum 500 dtn
Cyclic GMP EIA Standard 1 ea
581022-96SOLID
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Solid Plate ea
Potassium Hydroxide 1 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
Cyclic GMP AChE Tracer 100 dtn
Cyclic GMP EIA Antiserum 100 dtn
Cyclic GMP EIA Standard 1 ea
581022-96WELL
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Strip Plate 1 ea
Potassium Hydroxide 1 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
Cyclic GMP AChE Tracer 100 dtn
Cyclic GMP EIA Antiserum 100 dtn
Cyclic GMP EIA Standard 1 ea
581022-480WELL
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Strip Plate 5 ea
Potassium Hydroxide 1 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
Cyclic GMP AChE Tracer 500 dtn
Cyclic GMP EIA Antiserum 500 dtn
Cyclic GMP EIA Standard 1 ea
Size Price Quantity Subtotal
96 solid wells $270.00 $0.00
96 strip wells $270.00 $0.00
480 solid wells $1,013.00 $0.00
480 strip wells $1,013.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

  • All 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution.
  • Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay.

Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Cyclic GMP EIA Kit (without Acetic Anhydride) under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

Related Products

Cyclic AMP EIA Kit
Cyclic AMP EIA Kit (without Acetic Anhydride)
Cyclic GMP EIA Kit

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Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

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