Cortisol ELISA Kit

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Chemical structure definitions are available for many Cayman products. See Chemical Structure Database for details.

You may be eligible to receive a free sample Cortisol EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

Limit of detection: 12 pg/ml · Sensitivity: 50% B/B0: 70 pg/ml · Cortisol is a glucocorticoid produced by the adrenal cortex in response to adrenocorticotropic hormone (ACTH). It is secreted with a circadian periodicity, and peaks just prior to waking in the morning. Cortisol is often elevated in major depressive disorder, certain forms of hypertension, stress, AIDS, and in the visceral fat of obese individuals. Cortisol can be measured in many matrices including blood, urine, and saliva. In serum, approximately 90-95% of cortisol is bound to proteins. Urinary cortisol is not bound to proteins, but its levels are dependent on glomerular and tubular function. In saliva, approximately 67% of cortisol is unbound. There is generally good correlation between cortisol measurements in saliva and serum. Cayman Chemical’s Cortisol EIA Kit is a competitive assay that provides accurate measurements of cortisol within the range of 7.9-1,000 pg/ml · Each kit contains AChE tracer, antiserum, standard, Ellman’s reagent, EIA buffer, wash buffer, Tween 20, plates pre-coated with anti-rabbit IgG, antiserum and tracer dyes, plate cover, and complete instructions.

	Download Kit Booklet 794 Kb PDF
	Download Abbreviated Kit Protocol 424 Kb PDF
	Download Material Safety Data Sheet (MSDS) 172 Kb PDF
	Printer-friendly version of this page small PDF (usually less than 100 Kb)
	• US Letter paper 8½″ × 11″
	• A4 paper 21cm × 29.7cm

Many of our kits require a source of UltraPure Water. If you do not have a source it is available for purchase from Cayman Chemical. Please check the Additional Items Required section of this kit booklet to verify if Ultra Pure Water is needed. Please note that the 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution. Please see the individual kit booklets for these details or contact techserv@caymanchem.com.