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Limit of detection:
80% B/B0: ~19 pg/ml
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Sensitivity:
50% B/B0: ~129 pg/ml
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Estradiol (17β-estradiol) is the major estrogen secreted by the premenopausal ovary. Estrogens direct the development of the female genotype in embryogenesis and at puberty. In addition, estradiol is an important luteolytic agent in humans.1 Estradiol is synthesized from testosterone primarily in the ovarian granulosa cells and placenta, but small amounts can be produced in the adrenal gland.2,3 The conversion of testosterone to estradiol is accomplished by the aromatase system, which consists of 3 enzyme activities localized to the endoplasmic reticulum of the cells in these tissues.1,4 Plasma estradiol levels increase gradually between days 1-7 of the menstrual cycle followed by a sharp increase to a peak value of about 300 pg/ml on day 12 just prior to ovulation.1,5
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1
Erickson, G.F. The ovary: Basic principles and concepts. A. Physiology. 3rd ed., 973-1015 (1995).
2
Naka, M., Mais, D.E., Morinelli, T.A., et al. 7-[(1R,2S,3S,5R)-6,6-Dimethyl-3-(4-iodobenzenesulfonylamino)bicyclo[3.1.1]hept-2-yl]-5(Z)-heptenoic acid: A novel high-affinity radiolabeled antagonist for platelet thromboxane A2/prostaglandin H2 receptors. J Pharmacol Exp Ther 262 632-637 (1992).
3
Miller, W.L., Tyrrell, J.B. The adrenal cortex. 3rd edition, 555-711 (1995).
4
Vance, D.E. Cholesterol and related derivatives. 2nd ed., 725-748 (1988).
5
Munro, C.J., Stabenfeldt, G.H., Cragun, J.R., et al. Relationship of serum estradiol and progesterone concentrations to the excretion profiles of their major urinary metabolites as measured by enzyme immunoassay and radioimmunoassay. Clin Chem 37 838-844 (1991).
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