Estradiol ELISA Kit; β-Oestradiol EIA Kit; 17β-Estradiol EIA Kit; β-Estradiol EIA Kit

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Chemical structure definitions are available for many Cayman products. See Chemical Structure Database for details.

You may be eligible to receive a free sample Estradiol EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

Limit of detection: 80% B/B0: ~19 pg/ml · Sensitivity: 50% B/B0: ~129 pg/ml · Estradiol (17β-estradiol) is the major estrogen secreted by the premenopausal ovary. Estrogens direct the development of the female genotype in embryogenesis and at puberty. In addition, estradiol is an important luteolytic agent in humans.1 Estradiol is synthesized from testosterone primarily in the ovarian granulosa cells and placenta, but small amounts can be produced in the adrenal gland.2,3 The conversion of testosterone to estradiol is accomplished by the aromatase system, which consists of 3 enzyme activities localized to the endoplasmic reticulum of the cells in these tissues.1,4 Plasma estradiol levels increase gradually between days 1-7 of the menstrual cycle followed by a sharp increase to a peak value of about 300 pg/ml on day 12 just prior to ovulation.1,5

1  Erickson, G.F. The ovary: Basic principles and concepts. A. Physiology. 3rd ed., 973-1015 (1995). 2  Naka, M., Mais, D.E., Morinelli, T.A., et al. 7-[(1R,2S,3S,5R)-6,6-Dimethyl-3-(4-iodobenzenesulfonylamino)bicyclo[3.1.1]hept-2-yl]-5(Z)-heptenoic acid: A novel high-affinity radiolabeled antagonist for platelet thromboxane A2/prostaglandin H2 receptors. J Pharmacol Exp Ther 262 632-637 (1992). 3  Miller, W.L., Tyrrell, J.B. The adrenal cortex. 3rd edition, 555-711 (1995). 4  Vance, D.E. Cholesterol and related derivatives. 2nd ed., 725-748 (1988). 5  Munro, C.J., Stabenfeldt, G.H., Cragun, J.R., et al. Relationship of serum estradiol and progesterone concentrations to the excretion profiles of their major urinary metabolites as measured by enzyme immunoassay and radioimmunoassay. Clin Chem 37 838-844 (1991).

	Download Kit Booklet 800 Kb PDF
	Download Abbreviated Kit Protocol 427 Kb PDF
	Download Material Safety Data Sheet (MSDS) 175 Kb PDF
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Many of our kits require a source of UltraPure Water. If you do not have a source it is available for purchase from Cayman Chemical. Please check the Additional Items Required section of this kit booklet to verify if Ultra Pure Water is needed. Please note that the 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution. Please see the individual kit booklets for these details or contact techserv@caymanchem.com.