Limit of detection: 80% B/B₀: ~19 pg/ml · Sensitivity: 50% B/B₀: ~129 pg/ml · Estradiol (17β-estradiol) is the major estrogen secreted by the premenopausal ovary. Estrogens direct the development of the female genotype in embryogenesis and at puberty. In addition, estradiol is an important luteolytic agent in humans.¹ Estradiol is synthesized from testosterone primarily in the ovarian granulosa cells and placenta, but small amounts can be produced in the adrenal gland.^2,3 The conversion of testosterone to estradiol is accomplished by the aromatase system, which consists of 3 enzyme activities localized to the endoplasmic reticulum of the cells in these tissues.^1,4 Plasma estradiol levels increase gradually between days 1-7 of the menstrual cycle followed by a sharp increase to a peak value of about 300 pg/ml on day 12 just prior to ovulation.^1,5

1 Erickson, G.F. The ovary: Basic principles and concepts. A. Physiology. 3rd ed. 973-1015 (1995).

2 Naka, M., Mais, D.E., Morinelli, T.A., et al. 7-[(1R,2S,3S,5R)-6,6-Dimethyl-3-(4-iodobenzenesulfonylamino)bicyclo[3.1.1]hept-2-yl]-5(Z)-heptenoic acid: A novel high-affinity radiolabeled antagonist for platelet thromboxane A₂/prostaglandin H₂ receptors. J Pharmacol Exp Ther 262 632-637 (1992).

3 Miller, W.L., and Tyrrell, J.B. The adrenal cortex. 3rd edition 555-711 (1995).

4 Vance, D.E. Cholesterol and related derivatives. 2nd ed. 725-748 (1988).

5 Munro, C.J., Stabenfeldt, G.H., Cragun, J.R., et al. Relationship of serum estradiol and progesterone concentrations to the excretion profiles of their major urinary metabolites as measured by enzyme immunoassay and radioimmunoassay. Clin Chem 37 838-844 (1991).

Vance, D.E. Cholesterol and related derivatives. 2nd ed. 725-748 (1988).

Muñoz, N.M., Kim, K.P., Han, S.K., et al. Characterization of monoclonal antibodies specific for 14-kDa human group V secretory phospholipase A₂ (hVPLA2). Hybridoma 19 171-176 (2000).

Erickson, G.F. The ovary: Basic principles and concepts. A. Physiology. 3rd ed. 973-1015 (1995).

Naka, M., Mais, D.E., Morinelli, T.A., et al. 7-[(1R,2S,3S,5R)-6,6-Dimethyl-3-(4-iodobenzenesulfonylamino)bicyclo[3.1.1]hept-2-yl]-5(Z)-heptenoic acid: A novel high-affinity radiolabeled antagonist for platelet thromboxane A₂/prostaglandin H₂ receptors. J Pharmacol Exp Ther 262 632-637 (1992).

Munro, C.J., Stabenfeldt, G.H., Cragun, J.R., et al. Relationship of serum estradiol and progesterone concentrations to the excretion profiles of their major urinary metabolites as measured by enzyme immunoassay and radioimmunoassay. Clin Chem 37 838-844 (1991).

Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).

Pradelles, P., Grassi, J., and Maclouf, J.A. Enzyme immunoassays of eicosanoids using acetylcholinesterase as label: An alternative to radioimmunoassay. Anal Chem 57 1170-1173 (1985).

Maclouf, J., Grassi, J., and Pradelles, P. Development of enzyme-immunoassay techniques for the measurement of eicosanoids. 355-364 (1987).

Miller, W.L., and Tyrrell, J.B. The adrenal cortex. 3rd edition 555-711 (1995).

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