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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

Progesterone EIA Kit

Cayman Chemical Item Number 582601

Progesterone ELISA Kit

Progesterone EIA Kit

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Progesterone EIA Kit Analysis Tools

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Description

Limit of detection: 80% B/B0: 10 pg/ml · Sensitivity: 50% B/B0: 70 pg/ml · Progesterone, along with pregnenolone, is the biosynthetic precursor of all other steroid hormones. Progesterone is synthesized from cholesterol by the sequential action of desmolase in the mitochondria, which produces pregnenolone, followed by Δ4,5-isomerase in the outer mitochondrial membrane and smooth endoplasmic reticulum of steroid-secreting cells.1 The main function of progesterone is to prepare the uterine lining for implantation of a fertilized ovum and to maintain pregnancy. Measurement of serum or plasma progesterone levels are used as an index to monitor ovulation and investigate luteal function. Plasma concentrations of progesterone are approximately 0.2-0.8 ng/ml and 4-20 ng/ml during the follicular phase and luteal phase of the menstrual cycle, respectively.1 Salivary concentrations of progesterone are approximately 100-fold lower than those found in plasma.2

1 Erickson, G.F. The ovary: Basic principles and concepts. A. Physiology. 3rd ed. 973-1015 (1995).

2 Tallon, D.F., Gosling, J.P., Buckley, P.M., et al. Direct solid-phase enzyme immunoassay of progesterone in saliva. Clin Chem 30 1507-1511 (1984).

Synonyms
  • Progesterone ELISA Kit
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
Progesterone 100%
17β-Estradiol 7.2%
5β-Pregnan-3α-ol-20-one 6.7%
Pregnenolone 2.5%
17-hydroxy Progesterone 0.5%
Testosterone <0.05%
5α-Pregnan-3α,20α-diol <0.01%
5β-Pregnan-3α,20α-diol <0.01%
5β-Pregnan-3α,20α-diol glucuronide <0.01%
17α-Estradiol <0.01%
Estriol <0.01%
Estrone <0.01%
Promegestone <0.01%
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Background Reading

Tallon, D.F., Gosling, J.P., Buckley, P.M., et al. Direct solid-phase enzyme immunoassay of progesterone in saliva. Clin Chem 30 1507-1511 (1984).

Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).

Maclouf, J., Grassi, J., and Pradelles, P. Development of enzyme-immunoassay techniques for the measurement of eicosanoids. 355-364 (1987).

Pradelles, P., Grassi, J., and Maclouf, J.A. Enzyme immunoassays of eicosanoids using acetylcholinesterase as label: An alternative to radioimmunoassay. Anal Chem 57 1170-1173 (1985).

Erickson, G.F. The ovary: Basic principles and concepts. A. Physiology. 3rd ed. 973-1015 (1995).

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582601-480WELL
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Strip Plate 5 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
Progesterone AChE Tracer 500 dtn
Progesterone EIA Antiserum 500 dtn
Progesterone EIA Standard 1 ea
582601-480SOLID
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Solid Plate 5 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
Progesterone AChE Tracer 500 dtn
Progesterone EIA Antiserum 500 dtn
Progesterone EIA Standard 1 ea
582601-96SOLID
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Solid Plate 1 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
Progesterone AChE Tracer 100 dtn
Progesterone EIA Antiserum 100 dtn
Progesterone EIA Standard 1 ea
582601-96WELL
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Strip Plate 1 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
Progesterone AChE Tracer 100 dtn
Progesterone EIA Antiserum 100 dtn
Progesterone EIA Standard 1 ea
Size Price Quantity Subtotal
96 solid wells $185.00 $0.00
96 strip wells $185.00 $0.00
480 solid wells $694.00 $0.00
480 strip wells $694.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

  • All 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution.
  • Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay.

Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Progesterone EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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