Limit of detection: 80% B/B₀: 6 pg/ml · Sensitivity: 50% B/B₀: 32 pg/ml · Testosterone is the prototypic and predominant circulating androgenic steroid. It plays a major role in the growth and function of many reproductive and non-reproductive tissues and organs including muscle, liver, and brain, directing the development of the male phenotype during embryogenesis and at puberty. Testosterone is synthesized from 17α-hydroxy progesterone by the enzymes 17,20-lyase and 17β-hydroxysteroid dehydrogenase in the gonads of both males and females. In many target cells it is reduced to 5α-dihydro testosterone, which mediates many of the biological actions of testosterone. It is then further metabolized to 17β-estradiol by aromatase.¹ Serum levels of testosterone range from 0.5 ng/ml in women to approximately 6-10 ng/ml in men, declining with age.²

1 Vance, D.E. Cholesterol and related derivatives. 2^nd ed., 725-748 (1988).

2 Miller, W.L., Tyrrell, J.B. The adrenal cortex. 3^rd edition, 555-711 (1995).

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Maclouf, J., Grassi, J., Pradelles, P. Development of enzyme-immunoassay techniques for the measurement of eicosanoids. (1987).

Miller, W.L., Tyrrell, J.B. The adrenal cortex. 3^rd edition, 555-711 (1995).

Vance, D.E. Cholesterol and related derivatives. 2^nd ed., 725-748 (1988).

Maxey, K.M., Maddipati, K.R., Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).

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