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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

Testosterone EIA Kit

Cayman Chemical Item Number 582701

Testosterone ELISA Kit

Testosterone EIA Kit

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Testosterone EIA Kit Analysis Tools

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Pre-configured for analysis on MyAssays.com
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Description

Limit of detection: 80% B/B0: 6 pg/ml · Sensitivity: 50% B/B0: 32 pg/ml · Testosterone is the prototypic and predominant circulating androgenic steroid. It plays a major role in the growth and function of many reproductive and non-reproductive tissues and organs including muscle, liver, and brain, directing the development of the male phenotype during embryogenesis and at puberty. Testosterone is synthesized from 17α-hydroxy progesterone by the enzymes 17,20-lyase and 17β-hydroxysteroid dehydrogenase in the gonads of both males and females. In many target cells it is reduced to 5α-dihydro testosterone, which mediates many of the biological actions of testosterone. It is then further metabolized to 17β-estradiol by aromatase.1 Serum levels of testosterone range from 0.5 ng/ml in women to approximately 6-10 ng/ml in men, declining with age.2

1 Vance, D.E. Cholesterol and related derivatives. 2nd ed. 725-748 (1988).

2 Miller, W.L., and Tyrrell, J.B. The adrenal cortex. 3rd edition 555-711 (1995).

Synonyms
  • Testosterone ELISA Kit
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
19-Nortestosterone 140%
Testosterone 100%
5α-dihydro Testosterone 27.4%
5β-dihydro Testosterone 18.9%
Androstenedione 3.7%
11-keto Testosterone 2.2%
5-Androstenediol 0.51%
epi-Testosterone 0.2%
Progesterone 0.14%
Testosterone Enanthate 0.11%
Androsterone 0.05%
Androsterone Sulfate 0.04%
Testosterone Sulfate 0.03%
DHEA Sulfate 0.02%
Estradiol <0.01%
Testosterone Glucuronide <0.01%
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Background Reading

Pradelles, P., Grassi, J., and Maclouf, J.A. Enzyme immunoassays of eicosanoids using acetylcholinesterase as label: An alternative to radioimmunoassay. Anal Chem 57 1170-1173 (1985).

Maclouf, J., Grassi, J., and Pradelles, P. Development of enzyme-immunoassay techniques for the measurement of eicosanoids. 355-364 (1987).

Miller, W.L., and Tyrrell, J.B. The adrenal cortex. 3rd edition 555-711 (1995).

Vance, D.E. Cholesterol and related derivatives. 2nd ed. 725-748 (1988).

Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).

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582701-96WELL
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Strip Plate 1 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
Testosterone AChE Tracer 100 dtn
Testosterone EIA Antiserum 100 dtn
Testosterone EIA Standard 1 ea
582701-480WELL
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Strip Plate 5 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
Testosterone AChE Tracer 500 dtn
Testosterone EIA Antiserum 500 dtn
Testosterone EIA Standard 1 ea
582701-96SOLID
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Solid Plate 1 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
Testosterone AChE Tracer 100 dtn
Testosterone EIA Antiserum 100 dtn
Testosterone EIA Standard 1 ea
582701-480SOLID
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Solid Plate 5 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
Testosterone AChE Tracer 500 dtn
Testosterone EIA Antiserum 500 dtn
Testosterone EIA Standard 1 ea
Size Price Quantity Subtotal
96 solid wells $230.00 $0.00
96 strip wells $230.00 $0.00
480 solid wells $863.00 $0.00
480 strip wells $863.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

  • All 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution.
  • Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay.

Pricing updated 2012-02-10. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Testosterone EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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Estriol EIA Kit
Follicle-Stimulating Hormone EIA Kit New
Luteinizing Hormone EIA Kit
Progesterone EIA Kit
UltraPure Water
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