Limit of detection:
80% B/B0: 6 pg/ml
·
Sensitivity:
50% B/B0: 32 pg/ml
·
Testosterone is the prototypic and predominant circulating androgenic steroid. It plays a major role in the growth and function of many reproductive and non-reproductive tissues and organs including muscle, liver, and brain, directing the development of the male phenotype during embryogenesis and at puberty. Testosterone is synthesized from 17α-hydroxy progesterone by the enzymes 17,20-lyase and 17β-hydroxysteroid dehydrogenase in the gonads of both males and females. In many target cells it is reduced to 5α-dihydro testosterone, which mediates many of the biological actions of testosterone. It is then further metabolized to 17β-estradiol by aromatase.1 Serum levels of testosterone range from 0.5 ng/ml in women to approximately 6-10 ng/ml in men, declining with age.2
1
Vance, D.E. Cholesterol and related derivatives. 2nd ed. 725-748 (1988).
2
Miller, W.L., and Tyrrell, J.B. The adrenal cortex. 3rd edition 555-711 (1995).
Pradelles, P., Grassi, J., and Maclouf, J.A. Enzyme immunoassays of eicosanoids using acetylcholinesterase as label: An alternative to radioimmunoassay. Anal Chem 57 1170-1173 (1985).
Maclouf, J., Grassi, J., and Pradelles, P. Development of enzyme-immunoassay techniques for the measurement of eicosanoids. 355-364 (1987).
Miller, W.L., and Tyrrell, J.B. The adrenal cortex. 3rd edition 555-711 (1995).
Vance, D.E. Cholesterol and related derivatives. 2nd ed. 725-748 (1988).
Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).
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