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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

11-keto Testosterone EIA Kit

Cayman Chemical Item Number 582751

11-keto Testosterone ELISA Kit; 11-KT EIA Kit

11-keto Testosterone EIA Kit

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11-keto Testosterone EIA Kit Analysis Tools

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Description

Limit of detection: 80% B/B0: 1.3 pg/ml · Sensitivity: 50% B/B0: 5 pg/ml · While testosterone is the primary androgenic male steroid found in mammals, 11-keto testosterone (11-KT) is a second key androgenic steroid found in fish. It occurs in males together with testosterone in amounts which vary from less than 1 ng/ml to as much as 50-100 ng/ml, depending on the species and the stage of the reproductive cycle. In the sea bass, testosterone concentrations are generally higher than 11-KT, with peak levels found after the spawning season. 11-KT, on the other hand, remains at levels less than 1 ng/ml but rises abruptly to 4-6 ng/ml during spermiation at the height of the spawning season.1 11-KT also shows individual variations in Arctic char, with dominant males having significantly higher 11-KT levels.2

1 Prat, F., Zanuy, S., Carrillo, M., et al. Seasonal changes in plasma levels of gonadal steroids of sea bass, Dicentrarchus labrax L. Gen Comp Endocrinol 78 361-373 (1990).

2 Elofsson, U.O.E., Mayer, I., Damsgård, B., et al. Intermale competition in sexually mature arctic charr: effects on brain monoamines, endocrine stress responses, sex hormone levels, and behavior. Gen Comp Endocrinol 118 450-460 (2000).

Synonyms
  • 11-keto Testosterone ELISA Kit
  • 11-KT EIA Kit
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
11-keto Testosterone 100%
Adrenosterone 2.9%
4-Androsten-11β,17β-diol-3-one 0.01%
5α-Androstan-17β-ol-3-one <0.01%
5α-Androsten-3.beta,17.beta-diol <0.01%
Testosterone <0.01%
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Background Reading

Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).

Pradelles, P., Grassi, J., and Maclouf, J.A. Enzyme immunoassays of eicosanoids using acetylcholinesterase as label: An alternative to radioimmunoassay. Anal Chem 57 1170-1173 (1985).

Maclouf, J., Grassi, J., and Pradelles, P. Development of enzyme-immunoassay techniques for the measurement of eicosanoids. 355-364 (1987).

Prat, F., Zanuy, S., Carrillo, M., et al. Seasonal changes in plasma levels of gonadal steroids of sea bass, Dicentrarchus labrax L. Gen Comp Endocrinol 78 361-373 (1990).

Elofsson, U.O.E., Mayer, I., Damsgård, B., et al. Intermale competition in sexually mature arctic charr: effects on brain monoamines, endocrine stress responses, sex hormone levels, and behavior. Gen Comp Endocrinol 118 450-460 (2000).

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582751-480SOLID
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Solid Plate 5 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
11-keto Testosterone AChE Tracer 500 dtn
11-keto Testosterone EIA Antiserum 500 dtn
11-keto Testosterone EIA Standard 1 ea
582751-96WELL
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Strip Plate 1 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
11-keto Testosterone AChE Tracer 100 dtn
11-keto Testosterone EIA Antiserum 100 dtn
11-keto Testosterone EIA Standard 1 ea
582751-96SOLID
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Solid Plate 1 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
11-keto Testosterone AChE Tracer 100 dtn
11-keto Testosterone EIA Antiserum 100 dtn
11-keto Testosterone EIA Standard 1 ea
582751-480WELL
Precoated (Mouse Anti-Rabbit IgG) EIA 96-Well Strip Plate 5 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
11-keto Testosterone AChE Tracer 500 dtn
11-keto Testosterone EIA Antiserum 500 dtn
11-keto Testosterone EIA Standard 1 ea
Size Price Quantity Subtotal
96 solid wells $270.00 $0.00
96 strip wells $270.00 $0.00
480 solid wells $1,013.00 $0.00
480 strip wells $1,013.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

  • All 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution.
  • Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay.

Pricing updated 2012-02-11. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample 11-keto Testosterone EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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