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Please feel free to continue placing orders via our website or via fax at 734-971-3640. You may send an email to customer service at custserv@caymanchem.com , or to technical support at techserv@caymanchem.com which we will respond to the next business day. Cayman will resume regular business hours and shipping schedules on Tuesday, May 29, 2012. Thank you for your patience and understanding.

Endothelin EIA Kit

Cayman Chemical Item Number 583151

ET EIA Kit; Endothelin ELISA Kit

Endothelin EIA Kit

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Endothelin EIA Kit Analysis Tools

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Description

Limit of detection: 7.8 pg/ml · The endothelin peptide family consists of three isoforms, ET-1 (corresponding to the initially isolated and most predominant isoform), ET-2, and ET-3. ET-1 is a 21 amino acid peptide and is one of the most potent vasoconstritors currently known. ET-2 displays similar pharmacology to ET-1, whereas ET-3 is a weak vasoconstrictor but more potent inhibitor of platelet aggregation. Cayman’s Endothelin Assay is an immunometric (i.e., sandwich) EIA that permits endothelin measurements within the range of 0-250 pg/ml, typically with a limit of detection of 1.5 pg/ml. Inter- and intraassay CV’s of less than 10% may be achieved at most concentrations. This assay offers sensitive and specific analysis of endothelin in serum, plasma, urine, or cell culture media.
Synonyms
  • ET EIA Kit
  • Endothelin ELISA Kit
Stability 6 months
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
Endothelin-1 100%
Endothelin-2 100%
Endothelin-3 100%
VIC 100%
Big Endothelin 100%
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Background Reading

Lovenberg, W., and Miller, R.C. Endothelin: A review of its effects and possible mechanisms of action. Neurochem Res 15 407-417 (1990).

Karwatowska-Prokopczuk, E., and Wennmalm Å. Endothelium-derived constricting factor(s): The last novelty-endothelin. Clin Physiol 10 113-121 (1990).

Masaki, T., and Yanagisawa, M. Endothelins. Essays Biochem 27 79-89 (1992).

Créminon, C., Frobert, Y., Habib, A., et al. Enzyme immunometric assay for endothelin using tandem monoclonal antibodies. J Immunol Methods 162 179-192 (1993).

Gaston, R.S., Schlessinger, S.D., Sanders, P.W., et al. Cyclosporine inhibits the renal response to L-arginine in human kidney transplant recipients. J Am Soc Nephrol 5 1426-1433 (1995).

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583151-480WELL
Polysorbate 20 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
Human Plasma 500 dtn
Non-specific Mouse Serum 500 dtn
Endothelin AChE-FAb' Conjugate 500 dtn
Anti-Endothelin EIA Strip Plate 5 ea
Endothelin EIA Standard 1 ea
583151-96WELL
Polysorbate 20 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
Human Plasma 100 dtn
Non-specific Mouse Serum 100 dtn
Endothelin AChE-FAb' Conjugate 100 dtn
Anti-Endothelin EIA Strip Plate 1 ea
Endothelin EIA Standard 1 ea
Size Price Quantity Subtotal
96 wells $363.00 $0.00
480 wells $1,361.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

  • All 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution.
  • Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay.

Pricing updated 2012-05-26. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Endothelin EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

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