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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

Endothelin EIA Kit

Cayman Chemical Item Number 583151

ET EIA Kit; Endothelin ELISA Kit

Endothelin EIA Kit

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Description

Limit of detection: 7.8 pg/ml · The endothelin peptide family consists of three isoforms, ET-1 (corresponding to the initially isolated and most predominant isoform), ET-2, and ET-3. ET-1 is a 21 amino acid peptide and is one of the most potent vasoconstritors currently known. ET-2 displays similar pharmacology to ET-1, whereas ET-3 is a weak vasoconstrictor but more potent inhibitor of platelet aggregation. Cayman’s Endothelin Assay is an immunometric (i.e., sandwich) EIA that permits endothelin measurements within the range of 0-250 pg/ml, typically with a limit of detection of 1.5 pg/ml. Inter- and intraassay CV’s of less than 10% may be achieved at most concentrations. This assay offers sensitive and specific analysis of endothelin in serum, plasma, urine, or cell culture media.
Synonyms
  • ET EIA Kit
  • Endothelin ELISA Kit
Stability 6 months
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
Endothelin-1 100%
Endothelin-2 100%
Endothelin-3 100%
VIC 100%
Big Endothelin 100%
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Background Reading

Lovenberg, W., and Miller, R.C. Endothelin: A review of its effects and possible mechanisms of action. Neurochem Res 15 407-417 (1990).

Karwatowska-Prokopczuk, E., and Wennmalm Å. Endothelium-derived constricting factor(s): The last novelty-endothelin. Clin Physiol 10 113-121 (1990).

Masaki, T., and Yanagisawa, M. Endothelins. Essays Biochem 27 79-89 (1992).

Créminon, C., Frobert, Y., Habib, A., et al. Enzyme immunometric assay for endothelin using tandem monoclonal antibodies. J Immunol Methods 162 179-192 (1993).

Gaston, R.S., Schlessinger, S.D., Sanders, P.W., et al. Cyclosporine inhibits the renal response to L-arginine in human kidney transplant recipients. J Am Soc Nephrol 5 1426-1433 (1995).

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583151-480WELL
Polysorbate 20 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
Human Plasma 500 dtn
Non-specific Mouse Serum 500 dtn
Endothelin AChE-FAb' Conjugate 500 dtn
Anti-Endothelin EIA Strip Plate 5 ea
Endothelin EIA Standard 1 ea
583151-96WELL
Polysorbate 20 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
Human Plasma 100 dtn
Non-specific Mouse Serum 100 dtn
Endothelin AChE-FAb' Conjugate 100 dtn
Anti-Endothelin EIA Strip Plate 1 ea
Endothelin EIA Standard 1 ea
Size Price Quantity Subtotal
96 wells $363.00 $0.00
480 wells $1,361.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

  • All 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution.
  • Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay.

Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Endothelin EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

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