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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

Interleukin-1β (human) EIA Kit

Cayman Chemical Item Number 583311

Interleukin-1β (human) ELISA Kit; IL-1β (human) EIA Kit

Interleukin-1β (human) EIA Kit

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Description

Limit of detection: 3.9 pg/ml · Interleukin 1 (IL-1) is a name for a family of proteins which includes IL-1α, IL-1β, ILra (Interleukin receptor antagonist) and IL-18. IL-1β is synthesized as 269 amino acid pro-cytokine that is enzymatically cleaved by IL-1β-converting enzyme (ICE) into the active 154 amino acid, 17 kDa peptide mature peptide.1,2 IL-1β is produced by many cell types including macrophages, monocytes, astrocytes, endothelial cells, smooth muscle cells, keratinocytes, adipocytes, T cells, and eosinophils.3 Although IL-1α and IL-1β exhibit only 26% amino acid identity,1 they bind to the same cell-surface receptors, IL-1 RI and IL-1 RII, present on a variety of cell types involved in immune or inflammatory responses.4,5,6 Normal production of IL-1 is critical for mediating host responses to infection and injury.2,3 Disease states in which IL-1 actively participates include inflammatory diseases, such as arthritis, inflammatory bowel disease, and shock, as well as arteriosclerosis, allergic disease, and some types of cancer.3,7

1 March, C.J., Mosley, B., Larsen, A., et al. Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs. Nature 315 641-647 (1985).

2 Thornberry, N.A., Bull, H.G., Calaycay, J.R., et al. A novel heterodimeric cysteine protease is required for interleukin-1β processing in monocytes. Nature 356 768-774 (1992).

3 Dinarello, C.A. Interleukin-1 and the pathogenesis of the acute-phase response. N Engl J Med 311 1413-1418 (1984).

4 Sims, J.E., March, C.J., Cosman, D., et al. cDNA expression cloning of the IL-1 receptor, a member of the immunoglobulin superfamily. Science 241 585-589 (1988).

5 Sims, J.E., Acres, R.B., Grubin, C.E., et al. Cloning the interleukin 1 receptor from human T cells. Proc Natl Acad Sci USA 86 8946-8950 (1989).

6 McMahan, C.J., Slack, J.L., Mosley, B., et al. A novel IL-1 receptor, cloned from B cells by mammaliam expression, is expressed in many cell types. EMBO J 10(10) 2821-2832 (1991).

7 Rosenwasser, L.J. Biologic activities of IL-1 and its role in human disease. J Allergy Clin Immunol 102 344-350 (1998).

Synonyms
  • Interleukin-1β (human) ELISA Kit
  • IL-1β (human) EIA Kit
Stability 6 months
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
Interleukin-1β 100%
Interleukin-1α <0.01%
Interleukin-2 <0.01%

Background Reading

Grassi, J., Frobert, Y., Pradelles, P., et al. Production of monoclonal antibodies against interleukin-1α and -1β. Development of two enzyme immunometric assays (EIA) using acetylcholinesterase and their application to biological media. J Immunol Methods 123 193-210 (1989).

Grassi, J., Roberge, C.J., Frobert, Y., et al. Determination of IL1α, IL1β and IL2 in biological media using specific enzyme immunometric assays. Immunol Rev 119 125-142 (1991).

Gruaz, D., Didierjean, L., Grassi, J., et al. Interleukin 1α and β in psoriatic skin: Enzymoimmunoassay, immunoblot studies, and effect of systemic retinoids. Dermatologica 179 202-206 (1989).

583311-96WELL
Polysorbate 20 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
Human Plasma 100 dtn
Non-specific Mouse Serum 100 dtn
Interleukin-1β (human) AChE-FAb' Conjugate 100 dtn
Anti-Interleukin-1β (human) EIA Strip Plate 1 ea
Interleukin-1β (human) EIA Standard 1 ea
583311-480WELL
Polysorbate 20 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
Human Plasma 500 dtn
Non-specific Mouse Serum 500 dtn
Interleukin-1β (human) AChE-FAb' Conjugate 500 dtn
Anti-Interleukin-1β (human) EIA Strip Plate 5 ea
Interleukin-1β (human) EIA Standard 1 ea
Size Price Quantity Subtotal
96 wells $363.00 $0.00
480 wells $1,361.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

  • All 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution.
  • Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay.

Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Interleukin-1β (human) EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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