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Interleukin-4 (human) EIA Kit

Cayman Chemical Item Number 583341

Interleukin-4 (human) ELISA Kit; IL-4 (human) EIA Kit

Interleukin-4 (human) EIA Kit

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Description

Limit of detection: 15 pg/ml · Interleukin-4 (IL-4) is produced primarily by activated T-cells and mast cells. It is synthesized as a 153 amino acid peptide from which a 24 amino acid signal sequence is cleaved to form the mature protein.1 IL-4 increases proliferation of B-cells in response to LPS or anti-IgM, and increases the expression of class II MHC antigens, the IgE receptor, and the IL-4 receptor on B-cells. IL-4 inhibits NK cell activation and proliferation as well as IL-2-induced LAK activation.2 The biological effects of IL-4 are mediated by a high-affinity cell surface receptor complex consisting of α and γc subunits, of which the γc subunit is a component of the IL-2, IL-7, and IL-15 receptors and the α subunit which can be complexed with the IL-13α receptor.3,4,5,6 A soluble IL-4 receptor which binds IL-4 with high affinity in solution has been identified in human and mouse.7,8 Potent anti-tumor activity of IL-4 has been observed in murine tumor models.9 Cayman’s IL-4 (human) EIA Kit is an immunometric (i.e., sandwich) EIA that permits IL-4 measurements within the range of 0-2,000 pg/ml, typically with a limit of detection of 31 pg/ml. This assay provides a method for the sensitive, specific analysis of IL-4 in serum, plasma, or cell culture media.

1 Yokota, T., Otsuka, T., Mosmann, T., et al. Isolation and characterization of a human interleukin cDNA clone, homologous to mouse B-cell stimulatory factor 1, that express B-cell- and T-cell-stimulating activities. Proc Natl Acad Sci USA 83(16) 5894-5898 (1986).

2 Hillman, G.G., and Haas, G.P. Role of cytokines in lymphocyte functions. 37-54 (1995).

3 Kondo, M., Takeshita, T., Ishii, N., et al. Sharing of the interleukin-2 (IL-2) receptor γ chain between receptors for IL-2 and IL-4. Science 262 1874-1877 (1993).

4 Noguchi, M., Nakamura, Y., Russell, S.M., et al. Interleukin-2 receptor γ chain: A functional component of the interleukin-7 receptor. Science 262 1877-1880 (1993).

5 Zurawski, S.M., Chomarat, P., Djossou, O., et al. The primary binding subunit of the human interleukin-4 receptor is also a component of the interleukin-13 receptor. J Biol Chem 270(23) 13869-13878 (1995).

6 Hilton, D.J., Zhang, J., Metcalf, D., et al. Cloning and charcterization of a binding subunit of the interleukin 13 receptor that is also a component of the interleukin 4 receptor. Proc Natl Acad Sci USA 93 497-501 (2003).

7 Ruhl, S., Pluznik, D.H., and Feldman, G.M. Soluble interleukin-4 receptor production by murine myeloid progenitor cells: interleukin-6 and interferon-7. Cytokine 5(2) 144-149 (2003).

8 Fernandez-Botran, R., and Vitetta, E.S. A soluble, high-affinity, interleukin-4-binding protein is present in the biological fluids of mice. Proc Natl Acad Sci USA 87 4202-4206 (1990).

9 Tepper, R.I., Pattengale, P.K., and Leder, P. Murine interleukin-4 displays potent anti-tumor activity in vivo. Cell 57 503-512 (1989).

Synonyms
  • Interleukin-4 (human) ELISA Kit
  • IL-4 (human) EIA Kit
Stability 6 months
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
Interleukin-4 100%
Interleukin-1α <0.01%
Interleukin-1β <0.01%
Interleukin-2 <0.01%
Interleukin-6 <0.01%
Interleukin-8 <0.01%
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Background Reading

Yokota, T., Otsuka, T., Mosmann, T., et al. Isolation and characterization of a human interleukin cDNA clone, homologous to mouse B-cell stimulatory factor 1, that express B-cell- and T-cell-stimulating activities. Proc Natl Acad Sci USA 83(16) 5894-5898 (1986).

Hillman, G.G., and Haas, G.P. Role of cytokines in lymphocyte functions. 37-54 (1995).

Hilton, D.J., Zhang, J., Metcalf, D., et al. Cloning and charcterization of a binding subunit of the interleukin 13 receptor that is also a component of the interleukin 4 receptor. Proc Natl Acad Sci USA 93 497-501 (2003).

Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).

Zurawski, S.M., Chomarat, P., Djossou, O., et al. The primary binding subunit of the human interleukin-4 receptor is also a component of the interleukin-13 receptor. J Biol Chem 270(23) 13869-13878 (1995).

Grassi, J., Roberge, C.J., Frobert, Y., et al. Determination of IL1α, IL1β and IL2 in biological media using specific enzyme immunometric assays. Immunol Rev 119 125-142 (1991).

Kondo, M., Takeshita, T., Ishii, N., et al. Sharing of the interleukin-2 (IL-2) receptor γ chain between receptors for IL-2 and IL-4. Science 262 1874-1877 (1993).

Ruhl, S., Pluznik, D.H., and Feldman, G.M. Soluble interleukin-4 receptor production by murine myeloid progenitor cells: interleukin-6 and interferon-7. Cytokine 5(2) 144-149 (2003).

Fernandez-Botran, R., and Vitetta, E.S. A soluble, high-affinity, interleukin-4-binding protein is present in the biological fluids of mice. Proc Natl Acad Sci USA 87 4202-4206 (1990).

Noguchi, M., Nakamura, Y., Russell, S.M., et al. Interleukin-2 receptor γ chain: A functional component of the interleukin-7 receptor. Science 262 1877-1880 (1993).

Tepper, R.I., Pattengale, P.K., and Leder, P. Murine interleukin-4 displays potent anti-tumor activity in vivo. Cell 57 503-512 (1989).

Conrad, D.J., Kuhn, H., Mulkins, M., et al. Specific inflammatory cytokines regulate the expression of human monocyte 15-lipoxygenase. Proc Natl Acad Sci USA 89 217-221 (1992).

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583341-480WELL
Polysorbate 20 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
Human Plasma 500 dtn
Non-specific Mouse Serum 500 dtn
Interleukin-4 (human) AChE FAb' Conjugate 500 dtn
Anti-Interleukin-4 (human) EIA Strip Plate 5 ea
Interleukin-4 (human) EIA Standard 1 ea
583341-96WELL
Polysorbate 20 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
Human Plasma 100 dtn
Non-specific Mouse Serum 100 dtn
Interleukin-4 (human) AChE FAb' Conjugate 100 dtn
Anti-Interleukin-4 (human) EIA Strip Plate 1 ea
Interleukin-4 (human) EIA Standard 1 ea
Size Price Quantity Subtotal
96 wells $363.00 $0.00
480 wells $1,361.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

  • All 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution.
  • Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay.

Pricing updated 2012-05-26. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Interleukin-4 (human) EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

Related Products

Interleukin-1α (human) EIA Kit
Interleukin-2 (human) EIA Kit
Interleukin-6 (human) EIA Kit
TNF-α (human) EIA Kit
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