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Interleukin-6 (human) EIA Kit

Cayman Chemical Item Number 583361

Interleukin-6 (human) ELISA Kit; IL-6 (human) EIA Kit

Interleukin-6 (human) EIA Kit

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Interleukin-6 (human) EIA Kit Analysis Tools

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Description

Limit of detection: 7.8 pg/ml · Interleukin-6 (IL-6) is a 212 amino acid polypeptide produced by various cells including T-cells, monocytes and a number of tissues. The molecular weight varies from 21- 29 kDa due to extensive and variable phosphorylation and glycosylation.1 Post-translational modifications may be tissue specific and may also determine biological activity.2 IL-6 is involved in many defense mechanisms including the immune response, hematopoiesis, and acute phase reactions.3 IL-6 promotes the proliferation of activated B-cells, acts as a B-cell differentiation factor, and stimulates the secretion of immunoglobulins.3,4 IL-6 also induces the proliferation of other cells, including T-cells, mesangial cells, keratinocytes, Kaposi sarcoma cells, and synovial fibroblasts.5,6 Elevated levels of IL-6 have been detected in a number of disease states, including bacterial and viral infections, HIV infections, autoimmune diseases, and some cancers.7 Cayman’s IL-6 (human) EIA Kit is an immunometric (i.e., sandwich) EIA that permits IL-6 measurements within the range of 0-250 pg/ml, typically with a limit of detection of 7.8 pg/ml.

1 Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).

2 Grassi, J., Roberge, C.J., Frobert, Y., et al. Determination of IL1α, IL1β and IL2 in biological media using specific enzyme immunometric assays. Immunol Rev 119 125-142 (1991).

3 Wong, G.G., and Clark, S.C. Multiple actions of interleukin 6 within a cytokine network. Immunol Today 9 137-139 (1988).

4 Van Damme, J., Opdenakker, G., Simpson, R.J., et al. Identification of the human 26-kD protein, interferon β2 (IFN-β2), as a B cell hybridoma/plasmacytoma growth factor induced by interleukin 1 and tumor necrosis factor. J Exp Med 165 914-919 (1987).

5 Muraguchi, A., Hirano, T., Tang, B., et al. The essential role of B cell stimulatory factor 2 (BSF-2/IL-6) for the terminal differentiation of B cells. J Exp Med 167 332-344 (1988).

6 Giuili, G., Scholl, U., Bulle, F., et al. Molecular cloning of the cDNAs coding for the two subunits of soluble guanylyl cyclase from human brain. FEBS Lett 304 83-88 (1992).

7 Kishimoto, T. The biology of interleukin-6. Blood 74 1-10 (1989).

Synonyms
  • Interleukin-6 (human) ELISA Kit
  • IL-6 (human) EIA Kit
Stability 6 months
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
Interleukin-6 100%
G-GSF <0.01%
M-GSF <0.01%
Interleukin-1α <0.01%
Interleukin-1β <0.01%
Interleukin-2 <0.01%
TNFα <0.01%
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Background Reading

Grassi, J., Roberge, C.J., Frobert, Y., et al. Determination of IL1α, IL1β and IL2 in biological media using specific enzyme immunometric assays. Immunol Rev 119 125-142 (1991).

Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).

Van Damme, J., Opdenakker, G., Simpson, R.J., et al. Identification of the human 26-kD protein, interferon β2 (IFN-β2), as a B cell hybridoma/plasmacytoma growth factor induced by interleukin 1 and tumor necrosis factor. J Exp Med 165 914-919 (1987).

Muraguchi, A., Hirano, T., Tang, B., et al. The essential role of B cell stimulatory factor 2 (BSF-2/IL-6) for the terminal differentiation of B cells. J Exp Med 167 332-344 (1988).

Giuili, G., Scholl, U., Bulle, F., et al. Molecular cloning of the cDNAs coding for the two subunits of soluble guanylyl cyclase from human brain. FEBS Lett 304 83-88 (1992).

Tosato, G., Seamon, K.B., Goldman, N.D., et al. Monocyte-derived human B-cell growth factor identified as interferon-β2 (BSF-2, IL-6). Science 239 502-239 (1988).

Kishimoto, T. The biology of interleukin-6. Blood 74 1-10 (1989).

Wong, G.G., and Clark, S.C. Multiple actions of interleukin 6 within a cytokine network. Immunol Today 9 137-139 (1988).

Hirano, T., Akira, S., Taga, T., et al. Biological and clinical aspects of interleukin 6. Immunol Today 11 443-449 (1990).

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583361-96WELL
Polysorbate 20 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
Human Plasma 100 dtn
Non-specific Mouse Serum 100 dtn
Interleukin-6 (human) AChE-FAb' Conjugate 100 dtn
Anti-Interleukin-6 (human) EIA Strip Plate 1 ea
Interleukin-6 (human) EIA Standard 1 ea
583361-480WELL
Polysorbate 20 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
Human Plasma 500 dtn
Non-specific Mouse Serum 500 dtn
Interleukin-6 (human) AChE-FAb' Conjugate 500 dtn
Anti-Interleukin-6 (human) EIA Strip Plate 5 ea
Interleukin-6 (human) EIA Standard 1 ea
Size Price Quantity Subtotal
96 wells $363.00 $0.00
480 wells $1,361.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

  • All 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution.
  • Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay.

Pricing updated 2012-05-26. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Interleukin-6 (human) EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

Related Products

Interleukin-1α (human) EIA Kit
Interleukin-1β (human) EIA Kit
Interleukin-2 (human) EIA Kit
Interleukin-4 (human) EIA Kit
TNF-α (human) EIA Kit
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