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583751 Substance P EIA Kit
Substance P ELISA Kit
Substance P EIA Kit Workbook Links
Cayman offers a variety of analysis tools to assist you.
Chemical structure definitions are available for many Cayman products. See Chemical Structure Database for details.
You may be eligible to receive a free sample Substance P EIA Kit under the Cayman Challenge program.
Let Cayman analyze your samples for you. See EIA Service for details and availability.
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Limit of detection:
80% B/B0: 8.2 pg/ml
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Sensitivity:
50% B/B0: 32 pg/ml
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Substance P is a bioactive 12-amino acid peptide (Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-amide) first isolated in 1931 from brain and intestine. The peptide is involved in many physiological processes including pain modulation, smooth muscle contraction, blood pressure control, kidney function and water homeostasis.1,2,3 Substance P is widely distributed in numerous tissues and body fluids including the central and peripheral nervous system, gastrointestinal tract, respiratory tract, visual system and circulatory system.3 Serum levels of Substance P determined by EIA, after C18-SPE purification, ranges between 5-115 pg/ml with a mean of 38 pg/ml.4 These values are similar to those obtained by RIA analysis of Substance P in plasma of healthy adults.3 Cayman Chemical’s Substance P EIA is a competitive assay that provides accurate measurements of Substance P with a working range of 3.9 to 500 pg/ml, typically with an IC50 (50% B/B0) of ~30 pg/ml and IC80 (80% B/B0) of ~8 pg/ml. Inter- and intra-assay CV’s of less than 20% can be achieved at most concentrations of the standard curve. Purification of Substance P from serum by C-18 solid phase extraction (SPE) has been validated prior to assay using this kit.4
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1
Jessell, T.M. The role of substance P in sensory transmission and pain perception. (1981).
2
Leeman, S.E., Mroz, E.A. Substance P. Life Sci 15 2033-2044 (1974).
3
Pernow, B. Substance P. Pharmacol Rev 35(2) 85-141 (1983).
4
Fehder, W.P., Ho, W., Campbell, D.E., et al. Development and evaluation of a chromatographic procedure for partial purification of substance P with quantification by an enzyme immunoassay. Clinical and Diagnostic Laboratory Immunology 5 303-307 (1998).
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Purchase 583751 Substance P EIA Kit
Pricing is for North America only. Other customers should contact a distributor in their region.
This product is also available to buy in bulk quantities.
Please contact our
Sales Department
for a quote or to purchase.
Cayman strives to be a reliable biochemical reagent vendor by providing the best possible products and services.
To ask for assistance with one of our products please contact our Technical Help staff.
Warning This product is not for human or veterinary use.
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Pricing updated 2010-03-19.
Prices are subject to change without notice.
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Many of our kits require a source of UltraPure Water. If you do not have a source it is available for purchase from Cayman Chemical. Please check the Additional Items Required section of this kit booklet to verify if Ultra Pure Water is needed.
Please note that the 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution. Please see the individual kit booklets for these details or contact techserv@caymanchem.com.
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