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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

sPLA2 (human Type IIA) EIA Kit

Cayman Chemical Item Number 585000

sPLA2 (human Type IIa) ELISA Kit; Secretory Phospholipase A2 (human synovial) EIA Kit

sPLA2 (human Type IIA) EIA Kit

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sPLA2 (human Type IIA) EIA Kit Analysis Tools

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Description

Limit of detection: 15 pg/ml · sPLA2 are found in the venom of certain snakes (Types IA, IIA, and IIB from vipers, cobras, rattlesnakes, and kraits), in pancreatic juices (Type IB), in rat and murine testes (Type IIC), in placenta, synovial fluids and platelets (Type IIA), and in heart, placenta, lung, mast cells and P388D1 macrophages (Type V).1 Until 1994 the sPLA2 responsible for the release of arachidonic acid in inflammation was believed to be Type IIA. Although recently discovered isoforms of PLA2 clearly contribute to the release of arachidonic acid, sPLA2 (Type IIA) continues to be a protein of interest in the field of inflammation. This EIA is specific for Type IIA sPLA2, and does not crossreact with Type I, Type IV or Type V PLA2, or inflammatory mediators such as tumor necrosis factor, interleukin-1, or platelet activating factor. Cayman’s sPLA2 (human Type IIA) EIA Kit is a immunometric assay that can be used for quantification of sPLA2 in plasma, synovial fluid, and other sample matrices.

1 Dennis, E.A. The growing phospholipase A2 superfamily of signal transduction enzymes. Trends Biochem Sci 22 1-2 (1997).

Synonyms
  • sPLA2 (human Type IIa) ELISA Kit
  • Secretory Phospholipase A2 (human synovial) EIA Kit
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
sPLA2 (type IIA) 100%
Albumin <0.01%
Interleukin-1 <0.01%
PAF-AH <0.01%
cPLA2 (type IV) <0.01%
iPLA2 (type VI) <0.01%
Rat sPLA2 <0.01%
sPLA2 (type I) <0.01%
TNF <0.01%
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Background Reading

Grassi, J., Roberge, C.J., Frobert, Y., et al. Determination of IL1α, IL1β and IL2 in biological media using specific enzyme immunometric assays. Immunol Rev 119 125-142 (1991).

Dennis, E.A. The growing phospholipase A2 superfamily of signal transduction enzymes. Trends Biochem Sci 22 1-2 (1997).

Tischfield, J.A. A reassessment of the low molecular weight phospholipase A2 gene family in mammals. J Biol Chem 272 17247-17250 (1997).

585000-480WELL
Polysorbate 20 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
Non-specific Mouse Serum 500 dtn
sPLA2 (human Type IIA) AChE-FAb' Conjugate 500 dtn
Anti-sPLA2 (human Type IIA) EIA Strip Plate 5 ea
sPLA2 (human Type IIA) EIA Standard 1 ea
585000-96WELL
Polysorbate 20 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
Non-specific Mouse Serum 100 dtn
sPLA2 (human Type IIA) AChE-FAb' Conjugate 100 dtn
Anti-sPLA2 (human Type IIA) EIA Strip Plate 1 ea
sPLA2 (human Type IIA) EIA Standard 1 ea
Size Price Quantity Subtotal
96 wells $399.00 $0.00
480 wells $1,496.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample sPLA2 (human Type IIA) EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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sPLA2 (human Type IIA) Western Ready Control Exclusive
sPLA2 (mouse Type V) Polyclonal Antibody
sPLA2 (Type V) Inhibitor Screening Assay Kit
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