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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

TNF-α (human) EIA Kit

Cayman Chemical Item Number 589201

Tumor Necrosis Factor-α EIA Kit; TNF-α (human) ELISA Kit

TNF-α (human) EIA Kit

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Description

Limit of detection: 3.9 pg/ml · Tumor Necrosis Factor-α (TNF-α) is a 17 kDa polypeptide produced primarily by activated monocytes and macrophages. This polypeptide mediates many immune and inflammatory responses, including activation and differentiation of monocytes and macrophages, expression of MHC class I and II, and expression of adhesion molecules on endothelial cells.1,2,3 TNF-α is considered to be the primary mediator in many inflammatory conditions including toxic shock and sepsis.4,5 Cayman’s TNF-α assay is an immunometric (i.e., sandwich) EIA that permits TNF-α measurements within the range of 0-250 pg/ml, typically with a limit of detection of 1 pg/ml. Inter- and intra-assay CVs of less than 5% can be achieved at most concentrations. This assay allows sensitive, specific analysis of TNF-α in serum or plasma.

1 Vassalli, P. The pathophysiology of tumor necrosis factors. Annu Rev Immunol 10 411-452 (1992).

2 Pober, J.S., Lapierre, L.A., Stolpen, A.H., et al. Activation of cultured human endothelial cells by recombinant lymphotoxin: Comparison with tumor necrosis factor and interleukin 1 species. J Immunol 138 3319-3324 (1987).

3 Broudy, V.C., Harlan, J.M., and Adamson, J.W. Disparate effects of tumor necrosis factor-α/cachectin and tumor necrosis factor-β/lymphotoxin on hematopoietic growth factor production and neutrophil adhesion molecule expression by cultured human endothelial cells. J Immunol 138 4298-4302 (1987).

4 Beutler, B., Greenwald, D., Hulmes, J.D., et al. Identity of tumor necrosis factor and the macrophage-secreted factor cachectin. Nature 316 552-554 (1985).

5 Tracey, K.J., Beutler, B., Lowry, S.F., et al. Shock and tissue injury induced by recombinant human cachectin. Science 234 470-474 (1986).

Synonyms
  • Tumor Necrosis Factor-α EIA Kit
  • TNF-α (human) ELISA Kit
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
TNF-α 100%
TNF-β <0.01%
TNF-RI <0.01%
TNF-RII <0.01%
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Background Reading

Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).

Tracey, K.J., Beutler, B., Lowry, S.F., et al. Shock and tissue injury induced by recombinant human cachectin. Science 234 470-474 (1986).

Vassalli, P. The pathophysiology of tumor necrosis factors. Annu Rev Immunol 10 411-452 (1992).

Broudy, V.C., Harlan, J.M., and Adamson, J.W. Disparate effects of tumor necrosis factor-α/cachectin and tumor necrosis factor-β/lymphotoxin on hematopoietic growth factor production and neutrophil adhesion molecule expression by cultured human endothelial cells. J Immunol 138 4298-4302 (1987).

Grassi, J., Roberge, C.J., Frobert, Y., et al. Determination of IL1α, IL1β and IL2 in biological media using specific enzyme immunometric assays. Immunol Rev 119 125-142 (1991).

Pober, J.S., Lapierre, L.A., Stolpen, A.H., et al. Activation of cultured human endothelial cells by recombinant lymphotoxin: Comparison with tumor necrosis factor and interleukin 1 species. J Immunol 138 3319-3324 (1987).

Beutler, B., Greenwald, D., Hulmes, J.D., et al. Identity of tumor necrosis factor and the macrophage-secreted factor cachectin. Nature 316 552-554 (1985).

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589201-96WELL
Polysorbate 20 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
Human Plasma 100 dtn
Non-specific Mouse Serum 100 dtn
TNF-α (human) AChE-FAb' Conjugate 100 dtn
Anti-TNF-α (human) EIA Strip Plate 1 ea
TNF-α (human) EIA Standard 1 ea
589201-480WELL
Polysorbate 20 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
Human Plasma 500 dtn
Non-specific Mouse Serum 500 dtn
TNF-α (human) AChE-FAb' Conjugate 500 dtn
Anti-TNF-α (human) EIA Strip Plate 5 ea
TNF-α (human) EIA Standard 1 ea
Size Price Quantity Subtotal
96 wells $363.00 $0.00
480 wells $1,361.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample TNF-α (human) EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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