Angiotensin II EIA Kit
Cayman Chemical Item Number 589301
Angiotensin II ELISA Kit
Angiotensin II EIA Kit
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Angiotensin II EIA Kit Analysis Tools
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Description
Limit of detection:
1.5 pg/ml
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Angiotensin II is a primary reactive vasoconstrictor, the main stimulus for aldosterone release, and one of the causative factors of chronic hypertension. The active angiotensin II octapeptide is released via a tightly controlled series of prohormones and proteases as diagrammed below. Normal human plasma angiotensin II levels are from 10-30 pg/ml when measured at rest in the supine position; they increase on standing, exercise, dehydration, or sodium depletion. The unique, patented 'Immobilized Antigen' technology of this angiotensin II immunometric assay allows reliable detection of 1-2 pg/ml, or as little as 10% of the normal human plasma concentration.
| Synonyms |
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| Stability |
6 months |
| Storage |
-20°C |
| Shipping |
Wet ice
in continental US; may vary elsewhere
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| Specificity |
| Angiotensin II |
100% |
| Angiotensin III |
36% |
| Angiotensin 3-8 |
33% |
| Angiotensin I |
4% |
| Angiotensin 1-7 |
<0.01% |
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- All 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution.
- Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay.
Pricing updated 2012-02-12.
Prices are subject to change without notice.
To ask for assistance with one of our products please contact a Technical Support Scientist.
Warning This product is not for human or veterinary use.
You may be eligible to receive a free sample Angiotensin II EIA Kit under the Cayman Challenge program.
Let Cayman analyze your samples for you. See EIA Service for details and availability.
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Batch-specific Information
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FAQs
Is the Angiotensin II EIA Kit suitable for use with rat plasma and supernatant of cell culture?
The sequence for angiotensin II is conserved across mammalian species. Therefore, this kit will work with rat samples, however purification is required.
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Is purification needed for plasma samples prior to use in the Angiotensin II EIA Kit?
Yes, plasma samples need purification prior to use in the Angiotensin II EIA Kit. The chemicals are available from Sigma: o-Phenanthroline Catalog number 33510, p-hydroxymercuribenzoic acid Catalog number 55540 Pepstatin A Catalog number 77170. The phenyl cartridge is from Phenomenex/Strata. The catalog numbers are: 1 ml Cat 8B S006 EAK , 3 ml Cat 8B S006 HBJ.
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Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.
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