8-OH-dG ELISA Kit; DNA Damage EIA Kit

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Chemical structure definitions are available for many Cayman products. See Chemical Structure Database for details.

You may be eligible to receive a free sample 8-hydroxy-2-deoxy Guanosine EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

Limit of detection: 80% B/B0: 30 pg/ml · Sensitivity: 50% B/B0: 100 pg/ml · 8-hydroxy-2-deoxy Guanosine (8-OH-dG) is a product of oxidative damage of DNA by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress. Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors. Increased levels of 8-OH-dG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension. Cayman’s 8-OH-dG EIA is a competitive assay that can be used for the quantification of 8-OH-dG in urine, cell culture, plasma, and other sample matrices. The EIA utilizes an anti-mouse IgG-coated plate and a tracer consisting of an 8-OH-dG-enzyme conjugate. This format has the advantage of providing low variability and increased sensitivity compared to assays that utilize an antigen-coated plate. Our EIA typically displays IC50 (50% B/B0) and IC80 (80% B/B0) values of approximately 100 and 30 pg/ml, respectively. It is important to note that the 8-OH-dG antibody used in this assay recognizes both free 8-OH-dG and DNA-incorporated 8-OH-dG. Since complex samples such as plasma, cell lysates, and tissues are comprised of mixtures of DNA fragments and free 8-OH-dG, concentrations of 8-OH-dG reported by EIA methodology will not coincide with those reported by LC-MS where the single nucleoside is typically measured. This should be kept in mind when analyzing and interpreting experimental results.

	Download Kit Booklet 746 Kb PDF
	Download Abbreviated Kit Protocol 425 Kb PDF
	Download Material Safety Data Sheet (MSDS) 201 Kb PDF
	Printer-friendly version of this page small PDF (usually less than 100 Kb)
	• US Letter paper 8½″ × 11″
	• A4 paper 21cm × 29.7cm

Many of our kits require a source of UltraPure Water. If you do not have a source it is available for purchase from Cayman Chemical. Please check the Additional Items Required section of this kit booklet to verify if Ultra Pure Water is needed. Please note that the 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution. Please see the individual kit booklets for these details or contact techserv@caymanchem.com.