Limit of detection: 80% B/B₀: ~3 ng/ml · Sensitivity: 50% B/B₀: ~12 ng/ml · DNA methylation is an important epigenetic process regulating gene expression. Methylation occurs on carbon 5 of 2-deoxy cytidine yielding the modified base 5-methyl-2-deoxy cytidine. The methylation pattern of cells is tightly regulated during development with the methylation profile being transmitted from parent to daughter cells during cell division. Methylation results in long-term silencing of genes, while unmethylated regions of DNA can be actively transcribed. Global changes in methylation can be quantified by measuring plasma or urinary levels of 5-methyl-2-deoxy cytidine. These changes in methylation can provide valuable information about cancer status of an individual. For example, patients with leukemia excrete significantly elevated levels of 5-methyl-2-deoxy cytidine compared to healthy individuals. Global methylation within tissues can be measured in a similar manner, allowing study of tissue-specific changes that occur as a result of differentiation, aging, or carcinogenesis. Cayman’s DNA Methylation EIA Kit is a competitive assay that can be used for the quantification of 5-methyl-2-deoxy cytidine in urine, culture supernatants, plasma, and other sample matrices. The EIA typically displays IC₅₀ (50% B/B₀) and IC₈₀ (80% B/B₀) values of approximately 12 and 3 ng/ml, respectively.

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