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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

Histamine EIA Kit

Cayman Chemical Item Number 589651

1H-imidazole 4-ethaneamine; Histamine ELISA Kit

Histamine EIA Kit

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Description

Limit of detection: 80% B/B0: 1 nM · Sensitivity: 50% B/B0: 3.8 nM · Histamine (1H-imidazole 4-ethaneamine) is a component of the storage granules of myeloid cells including basophils1 and mast cells.2 Histamine secretion from gastric serosal cells contributes to the stimulation of gastric acid secretion. The clinical importance of histamine in the pathogenesis of allergy and asthma has long been appreciated.3 Together with the cysteinyl-leukotrienes, histamine contributes to the airway constriction, edema, vascular congestion, and inflammatory cell recruitment seen in acute allergic respiratory disease. Bacterial decarboxylation of the amino acid histidine in fresh fish produces histamine, which can be used as a health hazard biomarker indicative of bacterial contamination. The measurement of histamine levels is therefore of importance in fields as diverse as food quality inspection, pharmaceutical development, and clinical diagnostics. Our Histamine EIA kit is a derivitization-amplified competitive enzyme immunoassay which detects histamine within the range from 400 pg/ml to 50,000 pg/ml. The assay can be used for the analysis of histamine in blood (plasma or serum) without extraction or purification. The use of other sample types may require further processing or purification of the sample.

1 Peters, S.P., Kagey-Sobotka, A., MacGlashan, D.W., et al. Effect of prostaglandin D2 in modulating histamine release from human basophils. J Pharmacol Exp Ther 228 400-406 (1984).

2 Naclerio, R.M., Hubbard, W., Lichtenstein, L.M., et al. Clinical aspects of allergic disease. Origin of late phase histamine release.. J Allergy Clin Immunol 98 721-723 (1996).

3 Sydbom, A., Kumlin, M., and Dahlén, S. Stimulus-dependence and time-course of histamine and leukotriene release from chopped and dispersed human lung tissue. Agents Actions 20 198-201 (1987).

Synonyms
  • 1H-imidazole 4-ethaneamine
  • Histamine ELISA Kit
Stability 6 months
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
Histamine 100%
3 methyl Histamine 0.04%
1 methyl Histamine 0.01%
Histidine <0.01%
Serotonin <0.01%
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Background Reading

Sydbom, A., Kumlin, M., and Dahlén, S. Stimulus-dependence and time-course of histamine and leukotriene release from chopped and dispersed human lung tissue. Agents Actions 20 198-201 (1987).

Naclerio, R.M., Hubbard, W., Lichtenstein, L.M., et al. Clinical aspects of allergic disease. Origin of late phase histamine release.. J Allergy Clin Immunol 98 721-723 (1996).

Peters, S.P., Kagey-Sobotka, A., MacGlashan, D.W., et al. Effect of prostaglandin D2 in modulating histamine release from human basophils. J Pharmacol Exp Ther 228 400-406 (1984).
Size Price Quantity Subtotal
96 wells $422.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

  • All 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution.
  • Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay.

Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Histamine EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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