Cayman Chemical | Prostaglandin D Synthase (hematopoietic-type) FP-Based Inhibitor Screening Assay Kit - Green | Description • H-PGDS FP-Based Inhibitor Screening Assay Kit - Green; Prostaglandin D Synthase (hematopoietic-type) Fluorescence Polarization-Based Inhibitor Screening Assay Kit

600007 Prostaglandin D Synthase (hematopoietic-type) FP-Based Inhibitor Screening Assay Kit - Green

H-PGDS FP-Based Inhibitor Screening Assay Kit - Green; Prostaglandin D Synthase (hematopoietic-type) Fluorescence Polarization-Based Inhibitor Screening Assay Kit - Green

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• See more Fluorescence Polarization; High Throughput Screening; Assay Kits; Allergy/Asthma

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Prostaglandin D₂ (PGD₂) is synthesized by hematopoietic-type PGD-synthase (H-PGDS) in mast cells and is released in large quantities during allergic and asthmatic anaphylaxis. H-PGDS is therefore a key target for development of selective, potent inhibitors for therapeutic use against these diseases. Cayman’s H-PGDS FP-Based Inhibitor Screening Assay Kit - Green provides a rapid, accurate assay for screening H-PGDS inhibitors. In this assay, a H-PGDS inhibitor-fluorescein conjugate serves as a specific fluorescent probe for the enzyme. Displacement of the probe by any unlabeled H-PGDS inhibitor leads to a decrease in the fluorescence polarization (FP) state of the probe, providing a direct signal for binding of the inhibitor to the active site of the enzyme. This assay is robust (Z’ of 0.75) and eliminates the need for the traditional multistep assay that requires the use of highly unstable PGH₂. The assay has been validated using several known inhibitors of H-PGDS with IC₅₀ values ranging from nanomolar to millimolar concentrations.

	Download Kit Booklet 1138 Kb PDF
	Download Material Safety Data Sheet (MSDS) 63 Kb PDF
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	• US Letter paper 8½″ × 11″
	• A4 paper 21cm × 29.7cm

Purchase 600007 Prostaglandin D Synthase (hematopoietic-type) FP-Based Inhibitor Screening Assay Kit - Green

Pricing is for North America only. Other customers should contact a distributor in their region.

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Warning This product is not for human or veterinary use.

Pricing updated 2010-03-20.
Prices are subject to change without notice.