See personalized New Product recommendations! Get personalized New Product recommendations! Register or Login for personalized New Product recommendations!

Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

p53 Transcription Factor Assay Kit

Cayman Chemical Item Number 600020

p53 Transcription Factor Assay Kit

See more

Description

The tumor suppressor protein p53 plays a crucial role in coordinating cellular responses to genotoxic stress and holds many important clinical implications in the treatment of cancer. Cayman’s p53 Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts. A specific double-stranded DNA (dsDNA) sequence containing the p53 response element is immobilized onto the wells of a 96-well plate. p53 contained in a nuclear extract, binds specifically to the p53 response element and is detected by addition of a specific primary antibody directed against p53. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.
Stability 1 year
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Background Reading

Brooks, C.L., and Gu, W. p53 ubiquitination: Mdm2 and beyond. Mol Cell 21 307-315 (2006).

Haupt, S., Berger, M., Goldberg, Z., et al. Apoptosis-the p53 network. J Cell Sci 116 4077-4085 (2003).

Horn, H.F., and Vousden, K.H. Coping with stress: Multiple ways to activate p53. Oncogene 26 1306-1316 (2007).

Levine, A.J. p53, the cellular gatekeeper for growth and division. Cell 88 323-331 (1997).

Gasco, M., Shami, S., and Crook, T. The p53 pathway in breast cancer. Breast Cancer Res 4 70-76 (2002).

Dey, A., Verma, C.S., and Lane, D.P. Updates on p53: Modulation of p53 degradation as a therapeutic approach. Br J Cancer 98 4-8 (2008).

Royds, J.A., and Iacopetta, B. p53 and disease: When the guardian angel fails. Cell Death Differ 13 1017-1026 (2006).

Show all 7 Hide all but first 3
600020-96well
Transcription Factor Binding Assay Buffer (4X) 1 ea
Transcription Factor Antibody Binding Buffer (10X) 1 ea
Transcription Factor Developing Solution 1 ea
Transcription Factor Stop Solution 1 ea
Transcription Factor Reagent A 1 ea
Transcription Factor Goat Anti-Mouse HRP Conjugate 1 ea
Polysorbate 20 1 ea
Wash Buffer Concentrate (400X) 5 mL
Transcription Factor p53 96-Well Strip Plate 1 ea
Transcription Factor p53 Positive Control 1 ea
Transcription Factor p53 Primary Antibody 1 ea
Transcription Factor p53 Competitor dsDNA 1 ea
Size Price Quantity Subtotal
96 wells $410.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample p53 Transcription Factor Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

Related Products

ChREBP Transcription Factor Assay Kit
CREB (Phospho-Ser133) Transcription Factor Assay Kit
HIF-1α Transcription Factor Assay Kit
NF-κB (human p50/p65) Combo Transcription Factor Assay Kit
NF-κB (human p50) Transcription Factor Assay Kit
NF-κB (p65) Transcription Factor Assay Kit
Nuclear Extraction Buffer (2X)
Nuclear Extraction Hypotonic Buffer (10X)
Nuclear Extraction Kit
Nuclear Extraction PBS (10X)
Nuclear Extraction Phosphatase Inhibitors (50X)
Nuclear Extraction Protease Inhibitor Cocktail (100X)
p53 Designer Transcription Factor Assay Kit
p53DINP1 Polyclonal Antibody New
Pifithrin-μ
PPARα Transcription Factor Assay Kit
PPARα, δ, γ Complete Transcription Factor Assay Kit
PPARγ Transcription Factor Assay Kit
PPARδ Transcription Factor Assay Kit
SREBP-1 Transcription Factor Assay Kit
SREBP-2 Transcription Factor Assay Kit
Show all 21 Hide all but first 3

Downloads

Batch-specific Information

Login to access batch-specific information

Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

Other Resources
Management Team Company Profile Company History ChemAssistant Tools FAQs Cayman Europe Cayman Pharma CaBRI Cayman Gear Press Releases Illustrations and Charts Key Research Area Posters Article Library Analysis Tools Conference Schedule Order Terms Browser Recommendation Privacy Statement Site Map

Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA

Toll Free: (800) 364-9897 (USA and Canada Only) · Fax: (734) 971-3640

Copyright 2012 Cayman Chemical Company

Lost password?