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Holiday Notification: Cayman Chemical will be closed Monday, May 28, 2012, in observance of the Memorial Day holiday. More…
Please feel free to continue placing orders via our website or via fax at 734-971-3640. You may send an email to customer service at custserv@caymanchem.com , or to technical support at techserv@caymanchem.com which we will respond to the next business day. Cayman will resume regular business hours and shipping schedules on Tuesday, May 29, 2012. Thank you for your patience and understanding.

Hydrogen Peroxide Cell-Based Assay Kit

Cayman Chemical Item Number 600050

H2O2 Fluorometric Assay Kit

Hydrogen Peroxide Cell-Based Assay Kit

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Description

Hydrogen peroxide (H2O2) is a natural by-product of oxygen metabolism. It has strong oxidizing capacity and is thus considered a highly reactive oxygen species. It is well established that H2O2 is a cytotoxic agent but evidence also suggests that H2O2 may be an important regulator of eukaryotic signal transduction. Cayman’s H2O2 Cell-Based Assay Kit provides a simple fluorometric method for the sensitive quantitation of H2O2 in cultured cells. H2O2 is detected using 10-acetyl-3,7-dihydroxyphenoxazine (ADHP), a highly sensitive and stable probe for H2O2. In the presence of horseradish peroxidase, ADHP reacts with H2O2 with a 1:1 stoichiometry to produce highly fluorescent resorufin (excitation = 530-560 nm; emission = 590 nm). Catalase, an H2O2 scavenger, is included in the kit for checking specificity of the assay.
Synonyms
  • H2O2 Fluorometric Assay Kit
Stability 6 months
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Halliwell, B., Clement, M.V., and Long, L.H. Hydrogen peroxide in the human body. FEBS Lett 486 10-13 (2000).

Veal, E.A., Day, A.M., and Morgan, B.A. Hydrogen peroxide sensing and signaling. Mol Cell 26 (2007).

Rhee, S.G., Chang, T., Bae, Y.S., et al. Cellular regulation by hydrogen peroxide. J Am Soc Nephrol 14 S211-S215 (2003).

Zhou, M., Diwu, Z., Panchuk-Voloshina, N., et al. A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: Applications in detecting the activity of phagocyte NADPH oxidase and other oxidases. Anal Biochem 253 162-168 (1997).

Amundson, D.M., and Zhou, M. Fluorometric method for the enzymatic determination of cholesterol. J Biochem Biophys Meth 38 43-52 (1999).

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600050-96well
Cell-Based Assay Buffer Tablet 1 ea
Cell-Based Assay Hydrogen Peroxide Standard 1 ea
Cell-Based Assay Hydrogen Peroxide Detector ADHP 1 ea
Cell-Based Assay Horseradish Peroxidase 1 ea
Cell-Based Assay Catalase 1 ea
600050-480well
Cell-Based Assay Buffer Tablet 1 ea
Cell-Based Assay Hydrogen Peroxide Standard 1 ea
Cell-Based Assay Hydrogen Peroxide Detector ADHP 5 ea
Cell-Based Assay Horseradish Peroxidase 5 ea
Cell-Based Assay Catalase 5 ea
Size Price Quantity Subtotal
96 wells $81.00 $0.00
480 wells $304.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-26. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Hydrogen Peroxide Cell-Based Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

Related Products

10-Acetyl-3,7-dihydroxyphenoxazine
Antioxidant Assay Kit
Catalase Assay Kit
Hydrogen Peroxide (urinary) Assay Kit
Superoxide Dismutase Assay Kit
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Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

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Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA

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