600140  Autophagy/Cytotoxicity Dual Staining Kit
Click image to enlarge

• See below for pricing and ordering
• See analysis tools
• See more Cell-Based Assay; Fluorescence; High Throughput Screening; Assay Kits; Alzheimer's Research; Apoptosis; Cancer; Cell Biology; Drug Discovery; Endocrinology; Environmental; Inflammation; Obesity; Signal Transduction; Toxicology

Chemical structure definitions are available for many Cayman products. See Chemical Structure Database for details.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

Autophagy is a critical cellular process that involves the degradation and digestion of intracellular components by the lysosome. This process not only enables cells to efficiently mobilize and recycle cellular constituents, but also prevents the accumulation of damaged organelles, misfolded proteins, and invading microorganisms.1 Autophagy is a multi-step process that begins with the sequestration of cytoplasm, organelles, and proteins. These cellular components are sequestered by a double membrane, forming an autophagosome. The autophagosome then fuses with a lysosome to form an autolysosome, where the cellular material is then degraded.2,3 Normal autophagy is essential for survival, differentiation, development, and homeostasis. Dysregulation of autophagy has been implicated in cancer, infection, aging, and degenerative diseases.2 While autophagy most often acts to promote cell survival in response to stress, it can also promote cell death. The relationship between autophagy and apoptosis is complex. The two pathways share common stimuli, components, and can regulate the activity of each other.4 However, the specific factors and mechanisms that dictate the choice between autophagy and apoptosis remain unclear. Cayman’s Autophagy/Cytotoxicity Dual Staining Kit provides a convenient tool for studying the regulation of autophagy and cytotoxicity at the cellular level. The kit employs monodansylcadaverine (MDC), an autofluorescent substance incorporated into multilamellar bodies by both an ion trapping mechanism and the interaction with membrane lipids,5 as a probe for detection of autophagic vacuoles in cultured cells. Propidium iodide is used as a marker of cell death. Tamoxifen, a known inducer of autophagy, is included as a positive control.
1  Jin, S., White, E. Tumor suppression by autophagy through the management of metabolic stress. Autophagy 4(5) 563-566 (2008).
2  Levine, B., Kroemer, G. Autophagy in the pathogenesis of disease. Cell 132 27-42 (2008).
3  Munafó, D.B., Colombo, M.I. A novel assay to study autophagy: Regulation of autophagosome vacuole size by amino acid deprivation. J Cell Sci 114 3619-3629 (2001).
4  Mizushima, N., Levine, B., Cuervo, A.M., et al. Autophagy fights disease through cellular self-digestion. Nature 451 1069-1075 (2008).
5  Niemann, A., Takatsuki, A., Elsässer, H. The lysosomotropic agent monodansylcadaverine also acts as a solvent polarity probe. J Histochem Cytochem 48(2) 251-258 (2000).


Purchase 600140 Autophagy/Cytotoxicity Dual Staining Kit

Pricing is for North America only. Other customers should contact a distributor in their region.

This product is also available to buy in bulk quantities. Please contact our Sales Department for a quote or to purchase.

Cayman strives to be a reliable biochemical reagent vendor by providing the best possible products and services. To ask for assistance with one of our products please contact our Technical Help staff.

Warning This product is not for human or veterinary use.

Pricing updated 2010-03-19.
Prices are subject to change without notice.

  email: password: hide x LOGIN | REGISTER
Cayman Chemical
Topical Catalogs
Packed with articles, the latest products, and much more.

New
Eicosanoid

Allergy, Asthma, & Inflammation

Cancer

More...
Helping to make your research possible by providing quality biochemicals and assays for research in and much more.
toll free 800.364.9897
direct dial 734.971.3335
1180 East Ellsworth Road
Ann Arbor, Michigan 48108
USA
All contact info
© 2002-2010
Cayman Chemical