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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

Methionine Sulfoxide Immunoblotting Kit

Cayman Chemical Item Number 600160

MetO Immunoblotting Kit

Methionine Sulfoxide Immunoblotting Kit

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Description

Protein methionine sulfoxide (MetO) is a reversible oxidative modification that occurs by exposure of protein(s) methionine residues to reactive oxygen species (ROS).1,2 Methionine oxidation can alter the function(s) of the modified proteins and if not reversed by MetO reductases can be further oxidized to methionine sulfone, an irreversible modification.3 Methionine oxidation may occur as a by-product of phagocytotic oxidative bursts, normal mitochondrial respiration and may also occur from environmental chemical exposures.1,4,5 The overabundance of methionine sulfoxidation is implicated in age-related diseases.4 Cellular protein MetO levels may be influenced by the decrease or loss of methionine sulfoxide reductase (MSR) activity or by an overabundance of ROS leading to increased levels of dysfunctional proteins.5,6 The Methionine Sulfoxide Polyclonal Antibody provided in this assay kit detected IgG-containing MetO from sera of Alzheimer’s patients but not from normal control sera.2 Cayman’s MetO Immunoblotting Kit contains reagents needed for the immunochemical detection of proteins containing MetO residues by western blotting. MetO-containing samples of interest include those from cell or tissue lysates as well as semi-pure or purified proteins. Samples may be prepared with reducing or non-reducing sample buffer prior to SDS-PAGE and tested along side one SDS-PAGE well designated for the provided positive control. The MetO Polyclonal Antibody was isolated from rabbit serum generated after immunization with a oxidized corn protein (MetO-DZS18) rich in methionine.2 This polyclonal antibody is specific for protein methionine sulfoxide.

1 Stadtman, E.R., Van Remmen, H., Richardson, A., et al. Methionine oxidation and aging. Biochim Biophys Acta 1703 135-140 (2005).

2 Oien, D.B., Canello, T., Gabizon, R., et al. Detection of oxidized methionine in selected proteins, cellular extracts and blood serums by novel anti-methionine sulfoxide antibodies. Arch Biochem Biophys 485 35-40 (2009).

3 Moskovitz, J., Jenkins, N.A., Gilbert, D.J., et al. Chromosomal localization of the mammalian peptide-methionine sulfoxide reductase gene and its differential expression in various tissues. Proc Natl Acad Sci USA 93 3205-3208 (1996).

4 Berlett, B.S., and Stadtman, E.R. Protein oxidation in aging, disease, and oxidative stress. J Biol Chem 272(33) 20313-20316 (1997).

5 Cabreiro, F., Picot, C.R., Perichon, M., et al. Overexpression of mitochondrial methionine sulfoxide reductase B2 protects leukemia cells from oxidative stress-induced cell death and protein damage. J Biochem 283(24) 16673-16681 (2008).

6 Levine, R.L., Mosoni, L., Berlett, B.S., et al. Methionine residues as endogenous antioxidants in proteins. Proc Natl Acad Sci USA 93 15036-15040 (1996).

Synonyms
  • MetO Immunoblotting Kit
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Stadtman, E.R., Van Remmen, H., Richardson, A., et al. Methionine oxidation and aging. Biochim Biophys Acta 1703 135-140 (2005).

Cabreiro, F., Picot, C.R., Perichon, M., et al. Overexpression of mitochondrial methionine sulfoxide reductase B2 protects leukemia cells from oxidative stress-induced cell death and protein damage. J Biochem 283(24) 16673-16681 (2008).

Moskovitz, J., Jenkins, N.A., Gilbert, D.J., et al. Chromosomal localization of the mammalian peptide-methionine sulfoxide reductase gene and its differential expression in various tissues. Proc Natl Acad Sci USA 93 3205-3208 (1996).

Oien, D.B., Canello, T., Gabizon, R., et al. Detection of oxidized methionine in selected proteins, cellular extracts and blood serums by novel anti-methionine sulfoxide antibodies. Arch Biochem Biophys 485 35-40 (2009).

Levine, R.L., Mosoni, L., Berlett, B.S., et al. Methionine residues as endogenous antioxidants in proteins. Proc Natl Acad Sci USA 93 15036-15040 (1996).

Berlett, B.S., and Stadtman, E.R. Protein oxidation in aging, disease, and oxidative stress. J Biol Chem 272(33) 20313-20316 (1997).

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600160-1ea
Methionine Sulfoxide Polyclonal Antibody 1 ea
Methionine Sulfoxide Positive Control 1 ea
Methionine Sulfoxide IgG-Blocking Reagent 1 ea
Size Price Quantity Subtotal
10 blots $356.00 $0.00
Bulk Contact
Cart Total $0.00

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Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Methionine Sulfoxide Immunoblotting Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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