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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

Aldehyde Site (DNA and Protein) Detection Kit

Cayman Chemical Item Number 600170

Aldehyde Site (DNA and Protein) Detection Kit

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Description

Oxidative stress in cells can result in the appearance and accumulation of free aldehydes on DNA and proteins. Cayman’s Aldehyde Site (DNA and Protein) Detection Kit employs an aldehyde reactive probe (ARP), O-(biotinylcarbazoylmethyl) hydroxylamine, that reacts specifically with aldehyde groups resulting from protein or DNA modification. By using an excess amount of ARP, aldehyde sites in both protein and DNA can be converted to biotin-tagged aldehyde sites, which can be detected using avidin-conjugated reporters. Epigallocatechin Gallate (EGCG), a compound known to react with culture medium to generate hydrogen peroxide (H2O2) and cause DNA damage, is included to be used as a positive control. The kit is easy to use and can be easily adapted to high throughput screening for compounds imposing oxidative stress on cells or organisms.
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Cooke, M.S., Evans, M.D., Dizdaroglu, M., et al. Oxidative DNA damage: Mechanisms, mutation, and disease. FASEB J 17 1195-1214 (2003).

Poulsen, H.E., Weimann, A., and Loft, S. Methods to detect DNA damage by free radicals: Relation to excercise. Proc Nutr Soc 58 1007-1014 (1999).

Tachibana, H. Molecular basis for cancer chemoprevention by green tea polyphenol EGCG. Forum Nutr 61 156-169 (2009).

Nakamura, J., Walker, V.E., Upton, P.B., et al. Highly sensitive apurinic/apyrimidinic site assay can detect spontaneous and chemically induced depurination under physiological conditions. Cancer Res 58 222-225 (1998).

Elbling, L., Weiss, R., Teufelhofer, O., et al. Green tea extract and (−)-epigallocatechin-3-gallate, the major tea catechin, exert oxidant but lack antioxidant activities. FASEB J 19(7) 807-809 (2005).

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600170-96well
Cell-Based Assay Fixative 1 ea
Cell-Based Assay Epigallocatechin Gallate (EGCG) 1 ea
Aldehyde Site Assay Reactive Probe 120 µL
Aldehyde Site Assay Buffer (10X) 50 mL
Aldehyde Site Assay Blocking Solution 10 mL
Aldehyde Site Assay Denaturing Solution 2 × 5 mL
Aldehyde Site Assay Avidin-FITC Complex 1 ea
Size Price Quantity Subtotal
96 wells $254.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Aldehyde Site (DNA and Protein) Detection Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

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Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA

Toll Free: (800) 364-9897 (USA and Canada Only) · Fax: (734) 971-3640

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