Melanocortin-4 Receptor STEP Reporter Assay Kit (Luminescence)

Melanocortin-4 Receptor STEP Reporter Assay Kit (Luminescence) Exclusive

Cayman Chemical Item Number 600190

MC4R

Description

The melanocortin-4 receptor (MC4R) has important roles in weight regulation, sexual function, and inflammation. Mice deficient in MC4R have increased lipid deposition associated with elevated adiposity, while mutations in MC4R in humans are associated with early onset or severe obesity. This assay consists of a 96-well plate coated with both MC4R and Secretory Alkaline Phosphatase (SEAP) reporter constructs. Cells grown on the STEP complex will express MC4R at the cell surface. Binding of agonists to MC4R initiates a signaling cascade resulting in expression of SEAP. SEAP activity is measured following addition of a luminescence-based alkaline phosphatase substrate provided in the kit.

Synonyms

MC4R

Stability

1 year

Storage

-20°C

Shipping

Wet ice in continental US; may vary elsewhere

Background Reading

Govaerts, C., Srinivasan, S., Shapiro, A., et al. Obesity-associated mutations in the melanocortin 4 receptor provide novel insights into its function. Peptides 26 1909-1919 (2005).

MacKenzie, R.G. Obesity-associated mutations in the human melanocortin-4 receptor gene. Peptides 27 395-403 (2006).

Caquineau, C., Leng, G., Guan, X.M.M., et al. Effects of α-melanocyte-stimulating hormone on magnocellular oxytocin neurones and their activation at intromission in male rats. J Neuroendocrinol 18 685-691 (2006).

Lasaga, M., Debeljuk, L., Durand, D., et al. Role of α-melanocyte stimulating hormone and melanocortin 4 receptor in brain inflammation. Peptides 29 1825-1835 (2008).

Zhang, Y., Kilroy, G.E., Henagan, T.M., et al. Targeted deletion of melanocortin receptor subtypes 3 and 4, but not CART, alters nutrient partitioning and compromises behavioral and metabolic responses to leptin. FASEB J 19 1482-1491 (2005).

Van der Ploeg, L.H.T., Martin, W.J., Howard, A.D., et al. A role for the melanocortin 4 receptor in sexual function. Proc Natl Acad Sci USA 99(17) 11381-11386 (2002).

Wikberg, J.E.S., and Mutulis, F. Targeting melanocortin receptors: An approach to treat weight disorders and sexual dysfunction. Nat Rev Drug Discov 7 307-323 (2008).

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600190-1ea

96-Well Solid Plate (black) with lid