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Orexin 1 Receptor STEP Reporter Assay Kit (Luminescence) Exclusive

Cayman Chemical Item Number 600240

OX1R

Orexin 1 Receptor STEP Reporter Assay Kit (Luminescence)

See more	Cell-Based Assay (51) Chemiluminescence (5) High Throughput Screening (28) Assay Kits (320) STEP Assays (7) Alzheimer's Research (44) Atherosclerosis (323) Diabetes (134) Drug Discovery (76) Endocrinology (335) Hypertension (90) Metabolic Syndrome (66) Neuroscience (654) Obesity (162)

Description

The Orexin 1 Receptor (OX1R) may be an important therapeutic target for treatment of sleep disorders, obesity, emotional stress, and addiction. Cayman’s Orexin 1 Receptor STEP Reporter Assay Kit (Luminescence) consists of a 96-well plate coated with both OX1R and Secretory Alkaline Phosphatase (SEAP) reporter constructs (OX1R STEP Plate). Cells grown on the STEP complex will express OX1R at the cell surface. Binding of agonists to OX1R initiates a signal transduction cascade resulting in expression of SEAP which is secreted into the cell culture medium. Aliquots of culture medium are removed at time intervals beginning at about 24 hours and SEAP activity is measured following addition of a luminescence-based alkaline phosphatase substrate provided in the kit. The kit is easy to use and can be easily adapted to high throughput screening for therapeutic compounds regulating activation of OX1R. A known OX1R agonist, Orexin-A, is included in the kit for use as a positive control. The kit provides sufficient reagent to measure SEAP activity at three time points, using the black plates provided.

Synonyms	OX1R
Stability	1 year
Storage	-80°C
Shipping	Dry ice in continental US; may vary elsewhere

Background Reading

de Lecea, L., Kilduff, T.S., Peyron, C., et al. The hypocretins: Hypothalamus-specific peptides with neuroexcitatory activity. Proc Natl Acad Sci USA 95 322-327 (1998).

Sakurai, T., Amemiya, A., Ishii, M., et al. Orexins and orexin receptors: A family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior. Cell 92 573-585 (1998).

Rodgers, R.J., Ishii, Y., Halford, J.C.G., et al. Orexins and appetite regulation. Neuropeptides 36(5) 303-325 (2002).

Tsujino, N., and Sakurai, T. Orexin/hypocretin: A neuropeptide at the interface of sleep, energy homeostasis, and reward system. Pharmacol Rev 61 162-176 (2009).

Thannickal, T.C., Moore, R.Y., Nienhuis, R., et al. Reduced number of hypocretin neurons in human narcolepsy. Neuron 27 469-474 (2000).

Xie, X., Wisor, J.P., Hara, J., et al. Hypocretin/orexin and nociceptin/orphanin FQ coordinately regulate analgesia in a mouse model of stress-induced analgesia. J Clin Invest 118(7) 2471-2481 (2008).

Harris, G.C., Wimmer, M., and Aston-Jones, G. A role for lateral hypothalamic orexin neurons in reward seeking. Nature 437 556-559 (2005).

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600240-1ea
96-Well Solid Plate (black) with lid
SEAP Substrate (Luminescence)	15 mL
Orexin 1 Receptor STEP Strip Plate
Orexin-A Positive Control	10 µL

Size	Price	Quantity	Subtotal
100 tests	$400.00		$0.00
Bulk	Contact
		Cart Total	$0.00

Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Orexin 1 Receptor STEP Reporter Assay Kit (Luminescence) under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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Downloads

	Download Kit Booklet 574 Kb PDF
	Download Material Safety Data Sheet (MSDS) 324 Kb PDF
	Printer-friendly version of this page small PDF (usually less than 100 Kb)
	• US Letter paper 8½″ × 11″
	• A4 paper 21cm × 29.7cm
	Chemical structure definitions are available for many Cayman products. See Chemical Structure Database for details.

Batch-specific Information

Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

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