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Holiday Notification: Cayman Chemical will be closed Monday, May 28, 2012, in observance of the Memorial Day holiday. More…
Please feel free to continue placing orders via our website or via fax at 734-971-3640. You may send an email to customer service at custserv@caymanchem.com , or to technical support at techserv@caymanchem.com which we will respond to the next business day. Cayman will resume regular business hours and shipping schedules on Tuesday, May 29, 2012. Thank you for your patience and understanding.

Annexin V FITC Assay Kit

Cayman Chemical Item Number 600300

Annexin V FITC Assay Kit

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Description

One of the hallmarks of the early stages of apoptosis is the redistribution of membrane phospholipids such as phosphatidylserine and phosphatidylethanolamine from the inner to outer leaflet of the membrane bilayer where they are exposed on the cell surface. Externalization of phosphatidylserine residues to the outer plasma membrane leaflet allows their detection via their high affinity for annexin V, a phospholipid binding protein. Cayman’s Annexin V Assay Kit employs a FITC-conjugated Annexin V as a probe for phosphatidylserine on the outer membrane of apoptotic cells. Apoptotic cells bound with fluorochrome-labeled Annexin V can be visualized using fluorescence microscopy, flow cytometry, or a plate reader capable of fluorescence measurements. The reagents provided in the kit are sufficient to run 100 samples when using flow cytometry, or 500 samples when using a 96-well plate format.
Stability 1 year
Storage 4°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

van Engeland, M., Nieland, L.J.W., Ramaekers, F.C.S., et al. Annexin V-affinity assay: A review on an apoptosis detection system based on phosphatidylserine exposure. Cytometry 31 1-9 (1998).

Elmore, S. Apoptosis: A review of programmed cell death. Toxicol Pathol 35(4) 495-516 (2007).

Schiller, M., Bekerdjian-Ding, I., Heyder, P., et al. Autoantigens are translocated into small apoptotic bodies during early stages of apoptosis. Cell Death Differ 15 183-191 (2008).

600300-100 tests
Cell-Based Propidium Iodide Solution 1 ea
Cell-Based Annexin V FITC 50 µL
Cell-Based Assay Annexin V Binding Buffer (10X) 50 mL
Size Price Quantity Subtotal
100 tests $280.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-26. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Annexin V FITC Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

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