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Holiday Notification: Cayman Chemical will be closed Monday, May 28, 2012, in observance of the Memorial Day holiday. More…
Please feel free to continue placing orders via our website or via fax at 734-971-3640. You may send an email to customer service at custserv@caymanchem.com , or to technical support at techserv@caymanchem.com which we will respond to the next business day. Cayman will resume regular business hours and shipping schedules on Tuesday, May 29, 2012. Thank you for your patience and understanding.

Lysosome/Cytotoxicity Dual Staining Kit

Cayman Chemical Item Number 600310

Lysosome/Cytotoxicity Dual Staining Kit

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Description

Cayman’s Lysosome/Cytotoxicity Dual Staining Kit is a tool for studying lysosome function at the cellular level. The kit employs 4-nitro-7-(1-piperazinyl)-2,1,3-benzoxadiazole (NBD-PZ), which is membrane permeable and reacts with carboxylic acids in the acidic luminal environment of lysosomes,1 as a probe for the detection of lysosomes in cultured cells. Propidium iodide is used as a marker of cell death. Chloroquine, a known inhibitor of lysosome function, is included as a positive control. The kit provides sufficient reagents to effectively treat/stain up to 10 plates worth of cells irrespective of the number of wells/plate.

1 Ishiguro, K., Ando, T., and Goto, H. Novel application of 4-nitro-7-(1-piperazinyl)-2,1,3-benzoxadiazole to visualize lysosomes in live cells. Biotechniques 45(4) 465-468 (2008).

Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Fehrenbacher, N., and Jäättelä, M. Lysosomes as targets for cancer therapy. Cancer Res 65(8) 2993-2995 (2005).

Ishiguro, K., Ando, T., and Goto, H. Novel application of 4-nitro-7-(1-piperazinyl)-2,1,3-benzoxadiazole to visualize lysosomes in live cells. Biotechniques 45(4) 465-468 (2008).

Schneider, L., and Zhang, J. Lysosomal function in macromolecular homeostasis and bioenergetics in Parkinson’s disease. Mol Neurodegener 5 14 (2010).

Zhang, L., Sheng, R., and Qin, Z. The lysosome and neurodegenerative diseases. Acta Biochim Biophys Sin 41(6) 437-445 (2009).

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600310-1ea
Cell-Based Assay Buffer Tablet 5 ea
Cell-Based Propidium Iodide Solution 1 ea
Chloroquine Positive Control (25 mM) 1 ea
Shrimp Kit Box (96 Well)
NBD-PZ Stock Solution 100 µL
Size Price Quantity Subtotal
1 ea $198.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-26. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Lysosome/Cytotoxicity Dual Staining Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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