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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

Lysosome/Cytotoxicity Dual Staining Kit

Cayman Chemical Item Number 600310

Lysosome/Cytotoxicity Dual Staining Kit

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Description

Cayman’s Lysosome/Cytotoxicity Dual Staining Kit is a tool for studying lysosome function at the cellular level. The kit employs 4-nitro-7-(1-piperazinyl)-2,1,3-benzoxadiazole (NBD-PZ), which is membrane permeable and reacts with carboxylic acids in the acidic luminal environment of lysosomes,1 as a probe for the detection of lysosomes in cultured cells. Propidium iodide is used as a marker of cell death. Chloroquine, a known inhibitor of lysosome function, is included as a positive control. The kit provides sufficient reagents to effectively treat/stain up to 10 plates worth of cells irrespective of the number of wells/plate.

1 Ishiguro, K., Ando, T., and Goto, H. Novel application of 4-nitro-7-(1-piperazinyl)-2,1,3-benzoxadiazole to visualize lysosomes in live cells. Biotechniques 45(4) 465-468 (2008).

Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Fehrenbacher, N., and Jäättelä, M. Lysosomes as targets for cancer therapy. Cancer Res 65(8) 2993-2995 (2005).

Schneider, L., and Zhang, J. Lysosomal function in macromolecular homeostasis and bioenergetics in Parkinson’s disease. Mol Neurodegener 5 14 (2010).

Ishiguro, K., Ando, T., and Goto, H. Novel application of 4-nitro-7-(1-piperazinyl)-2,1,3-benzoxadiazole to visualize lysosomes in live cells. Biotechniques 45(4) 465-468 (2008).

Zhang, L., Sheng, R., and Qin, Z. The lysosome and neurodegenerative diseases. Acta Biochim Biophys Sin 41(6) 437-445 (2009).

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600310-1ea
Cell-Based Assay Buffer Tablet 5 ea
Cell-Based Propidium Iodide Solution 1 ea
Chloroquine Positive Control (25 mM) 1 ea
Shrimp Kit Box (96 Well)
NBD-PZ Stock Solution 100 µL
Size Price Quantity Subtotal
1 ea $198.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Lysosome/Cytotoxicity Dual Staining Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

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