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Holiday Notification: Cayman Chemical will be closed Monday, May 28, 2012, in observance of the Memorial Day holiday. More…
Please feel free to continue placing orders via our website or via fax at 734-971-3640. You may send an email to customer service at custserv@caymanchem.com , or to technical support at techserv@caymanchem.com which we will respond to the next business day. Cayman will resume regular business hours and shipping schedules on Tuesday, May 29, 2012. Thank you for your patience and understanding.

Multi-Parameter Apoptosis Assay Kit

Cayman Chemical Item Number 600330

Multi-Parameter Apoptosis Assay Kit

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Description

Cellular events in apoptosis include loss of cell membrane asymmetry, cell shrinkage, membrane blebbing, nuclear fragmentation, and chromatin condensation. Cayman’s Multi-Parameter Apoptosis Assay Kit includes four different reagents for monitoring several events in apoptosis. It employs FITC-conjugated Annexin V as a probe for phosphatidylserine on the outer membrane of apoptotic cells, TMRE as a probe for mitochondrial membrane potential, 7-AAD as an indicator of membrane permeability/cell viability, and Hoechst Dye to demonstrate nuclear morphology. The kit allows phenotypic characterization of different cell death parameters at a single-cell level. The assay can be adapted to high content screening with appropriate equipment. The reagents provided in the kit are sufficient to run 100 samples when using flow cytometry, or 500 samples when using a 96-well plate format.
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Schiller, M., Bekerdjian-Ding, I., Heyder, P., et al. Autoantigens are translocated into small apoptotic bodies during early stages of apoptosis. Cell Death Differ 15 183-191 (2008).

van Engeland, M., Nieland, L.J.W., Ramaekers, F.C.S., et al. Annexin V-affinity assay: A review on an apoptosis detection system based on phosphatidylserine exposure. Cytometry 31 1-9 (1998).

Elmore, S. Apoptosis: A review of programmed cell death. Toxicol Pathol 35(4) 495-516 (2007).

600330-1ea
Shrimp Kit Box (96 Well)
Cell-Based Assay 7-AAD Staining Stock Solution (1,000X) 1 ea
Cell-Based Annexin V FITC 50 µL
Cell-Based Assay Annexin V Binding Buffer (10X) 50 mL
Cell-Based Assay TMRE 100 µL
Cell-Based Assay Hoechst Dye 50 µL
Size Global Purchasing
5 x 96 wells See a distributor in your region for pricing

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Multi-Parameter Apoptosis Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

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