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COX-2 (ovine)

Cayman Chemical Item Number 60120

Prostaglandin H Synthase 2; Inducible cyclooxygenase (ovine); Cyclooxygenase 2

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Description

Mr: 72.00 · Specific activity: >3,000 units/mg · Enzyme Commission (EC): 1.14.99.1 · Isolated from sheep placenta · One unit of enzyme consumes one nmol of oxygen per minute at 37°C in 0.1 M Tris-HCl buffer, pH 8.0, containing 100 µM arachidonate, 5 mM EDTA, 2 mM phenol, and 1 µM hematin.1,2

1 Johnson, J.L., Wimsatt, J., Buckel, S.D., et al. Purification and characterization of prostaglandin H synthase-2 from sheep placental cotyledons. Arch Biochem Biophys 324 26-34 (1995).

2 Capdevila, J.H., Morrow, J.D., Belosludtsev, Y.Y., et al. The catalytic outcomes of the constitutive and the mitogen inducible isoforms of prostaglandin H2 synthase are markedly affected by glutathione and glutathione peroxidase(s). Biochemistry 34 3325-3337 (1995).

Synonyms
  • Prostaglandin H Synthase 2
  • Inducible cyclooxygenase (ovine)
  • Cyclooxygenase 2
Formulation A solution in 80 mM Tris-HCl, pH 8.0, containing 0.1% Tween 20 and 300 µM DDC
Purity ~70%
Stability 1 year
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Background Reading

Xie, W., Chipman, J.G., Robertson, D.L., et al. Expression of a mitogen-responsive gene encoding prostaglandin synthase is regulated by mRNA splicing. Proc Natl Acad Sci USA 88 2692-2696 (1991).

Capdevila, J.H., Morrow, J.D., Belosludtsev, Y.Y., et al. The catalytic outcomes of the constitutive and the mitogen inducible isoforms of prostaglandin H2 synthase are markedly affected by glutathione and glutathione peroxidase(s). Biochemistry 34 3325-3337 (1995).

Claesson, H., and Feinmark, S.J. Relationship of cyclic-AMP levels in leukotriene B4-stimulated leukocytes to lysosomal enzyme release and the generation of superoxide anions. Biochim Biophys Acta 804 52-57 (1984).

Johnson, J.L., Wimsatt, J., Buckel, S.D., et al. Purification and characterization of prostaglandin H synthase-2 from sheep placental cotyledons. Arch Biochem Biophys 324 26-34 (1995).

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Size Price Quantity Subtotal
1 Kunits $115.00 $0.00
5 Kunits $518.00 $0.00
10 Kunits $920.00 $0.00
Bulk Contact
Cart Total $0.00

This product is available in custom sizes and/or larger quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-26. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

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