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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

COX-2 (human recombinant)

Cayman Chemical Item Number 60122

Prostaglandin H Synthase 2; Cyclooxygenase 2

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Description

Source: recombinant N-terminal hexahistidine-tagged enzyme isolated from a Baculovirus overexpression system in Sf21 cells · Mr: 70.00 · Specific activity: >8,000 units/mg · Recombinant enzyme isolated from a Baculovirus overexpression system in Sf21 cells · One unit of enzyme consumes one nmol of oxygen per minute at 37°C in 0.1 M Tris-HCl buffer (pH 8.0), containing 100 µM arachidonate, 5 mM EDTA, 2 mM phenol, and 1 µM hematin.
Synonyms
  • Prostaglandin H Synthase 2
  • Cyclooxygenase 2
Formulation 80 mM Tris-HCl, pH 8.0, with 0.1% polysorbate 20 and 300 μM DDC
Purity >70%
Stability 6 months
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Background Reading

Hla, T., and Neilson, K. Human cyclooxygenase-2 cDNA. Proc Natl Acad Sci USA 89 7384-7388 (1992).

Cromlish, W.A., and Kennedy, B.P. Selective inhibition of cyclooxygenase-1 and -2 using intact insect cell assays. Biochem Pharmacol 52 1777-1785 (1996).

Smith, T., Leipprandt, J., and Dewitt, D. Purification and characterization of the human recombinant histidine-tagged prostaglandin endoperoxide H Synthases-1 and -2. Arch Biochem Biophys 375 195-200 (2000 Jan 1).

Jones, D.A., Carlton, D.P., McIntyre, T.M., et al. Molecular cloning of human prostaglandin endoperoxide synthase type II and demonstration of expression in response to cytokines. J Biol Chem 268 9049-9054 (1993).

Xie, W., Chipman, J.G., Robertson, D.L., et al. Expression of a mitogen-responsive gene encoding prostaglandin synthase is regulated by mRNA splicing. Proc Natl Acad Sci USA 88 2692-2696 (1991).

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Size Price Quantity Subtotal
1 Kunits $154.00 $0.00
2.5 Kunits $366.00 $0.00
5 Kunits $693.00 $0.00
Bulk Contact
Cart Total $0.00

This product is available in custom sizes and/or larger quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-02-11. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

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