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Please feel free to continue placing orders via our website or via fax at 734-971-3640. You may send an email to customer service at custserv@caymanchem.com , or to technical support at techserv@caymanchem.com which we will respond to the next business day. Cayman will resume regular business hours and shipping schedules on Tuesday, May 29, 2012. Thank you for your patience and understanding.

5-Lipoxygenase (human recombinant)

Cayman Chemical Item Number 60402

5-LO; 5-LOX

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Description

Source: active recombinant enzyme expressed in Sf21 cells · Mr: 78 kDa · Enzyme Commission (EC): 1.13.11.34 · Recombinant enzyme isolated from a Baculovirus overexpression system in Sf21 cells · Avoid repeated thawing and refreezing · One unit of enzyme consumes one nmol of oxygen per minute at 25°C in 50 mM Tris-HCl buffer, pH 7.5, containing 100 µM arachidonate, 2 mM CaCl2, 1 mM ATP.1,2

1 Zhang, Y., Lind, B., Rådmark, O., et al. Iron content of human 5-lipoxygenase, effects of mutations regarding conserved histidine residues. J Biol Chem 268 2535-2541 (1993).

2 Zhang, Y.Y., Rådmark, O., and Samuelsson, B. Mutagenesis of some conserved residues in human 5-lipoxygenase: Effects on enzyme activity. Proc Natl Acad Sci USA 89 485-489 (1992).

Synonyms
  • 5-LO
  • 5-LOX
Formulation A solution in 100 mM Tris-HCl, pH 8.0, containing 5 mM EGTA, 1mM CaCl2, and 30% glycerol
Purity cell ly100,000 x g supernatant
Stability 6 months
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Background Reading

Zhang, Y.Y., Rådmark, O., and Samuelsson, B. Mutagenesis of some conserved residues in human 5-lipoxygenase: Effects on enzyme activity. Proc Natl Acad Sci USA 89 485-489 (1992).

Peters-Golden, M. Cell biology of the 5-lipoxygenase pathway. Am J Respir Crit Care Med 157 5227-5232 (1998).

Zhang, Y., Lind, B., Rådmark, O., et al. Iron content of human 5-lipoxygenase, effects of mutations regarding conserved histidine residues. J Biol Chem 268 2535-2541 (1993).

Komatsu, N., Natsui, K., Ueda, N., et al. Immunohistochemical study on arachidonate 5-lipoxygenase in porcine leukocytes and other tissues. J Histochem Cytochem 39 655-662 (1991).

Brock, T.G., McNish, R.W., Bailie, M.B., et al. Rapid import of cytosolic 5-lipoxygenase into the nucleus of neutrophils after in vivo recruitment and in vitro adherence. J Biol Chem 272 8276-8280 (1997).

Pouliot, M., McDonald, P.P., Krump, E., et al. Colocalization of cytosolic phospholipase A2, 5-lipoxygenase, and 5-lipoxygenase-activating protein at the nuclear membrane of A23187-stimulated human neutrophils. Eur J Biochem 238 250-258 (1996).

Shimizu, T., Rådmark, O., and Samuelsson, B. Enzyme with dual lipoxygenase activities catalyzes leukotriene A4 synthesis from arachidonic acid. Proc Natl Acad Sci USA 81 689-693 (1984).

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Size Price Quantity Subtotal
500 units $81.00 $0.00
1 Kunit $154.00 $0.00
2.5 Kunit $365.00 $0.00
5 Kunit $648.00 $0.00
Bulk Contact
Cart Total $0.00

This product is available in custom sizes and/or larger quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-26. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

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