M_r: 94.00 · Specific activity: >1 million units/mg · Enzyme Commission (EC): 1.13.11.33 · Isolated from soybeans (Provar) · One unit of enzyme causes an increase of 0.001 absorbance units per minute at 234 nm when incubated with 0.02% linoleate at 25°C in 0.1 M borate buffer, pH 9.0, in a total volume of 1.0 ml.^1,2,3,4

1 Draheim, J.E., Carroll, R.T., McNemar, T.B., et al. Lipoxygenase isoenzymes: A spectroscopic and structural characterization of soybean seed enzymes. Arch Biochem Biophys 269 208-218 (1989).

2 Funk, M.O., Whitney, M.A., Hausknecht, E.C., et al. Resolution of the isoenzymes of soybean lipoxygenase using isoelectric focusing and chromatofocusing. Anal Biochem 146 246-251 (1985).

3 Boyington, J.C., Gaffney, B.J., and Amzel, L.M. The three-dimensional structure of an arachidonic acid 15-lipoxygenase. Science 260 1482-1486 (1993).

4 Gaffney, B.J., Mavrophilipos, D.V., and Doctor, K.S. Access of ligands to the ferric center in lipoxygenase-1. Biophys J 64 773-783 (1993).

Draheim, J.E., Carroll, R.T., McNemar, T.B., et al. Lipoxygenase isoenzymes: A spectroscopic and structural characterization of soybean seed enzymes. Arch Biochem Biophys 269 208-218 (1989).

Funk, M.O., Whitney, M.A., Hausknecht, E.C., et al. Resolution of the isoenzymes of soybean lipoxygenase using isoelectric focusing and chromatofocusing. Anal Biochem 146 246-251 (1985).

Gaffney, B.J., Mavrophilipos, D.V., and Doctor, K.S. Access of ligands to the ferric center in lipoxygenase-1. Biophys J 64 773-783 (1993).

Boyington, J.C., Gaffney, B.J., and Amzel, L.M. The three-dimensional structure of an arachidonic acid 15-lipoxygenase. Science 260 1482-1486 (1993).

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