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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

iNOS (mouse recombinant)

Cayman Chemical Item Number 60864

NOS II; Inducible Nitric Oxide Synthase

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Description

Mr: 130.00 kDa · Enzyme Commission (EC): 1.14.13.39 · Recombinant enzyme expressed in E. coli · One unit of enzyme produces 1 nmol of NO per minute at 37°C in 50 mM HEPES, pH 7.4, containing 1 mM arginine, 1 mM magnesium acetate, 5 µM oxyhemoglobin, 0.1 mM NADPH, 12 µM tetrahydrobiopterin, and 170 µM DTT.1,2

1 Hevel, J.M., and Marletta, M.A. Nitric-oxide synthase assays. Methods Enzymol 233 250-258 (1994).

2 Lyons, C.R., Orloff, G.J., and Cunningham, J.M. Molecular cloning and functional expression of an inducible nitric oxide synthase from a murine macrophage cell line. J Biol Chem 267 6370-6374 (1992).

Synonyms
  • NOS II
  • Inducible Nitric Oxide Synthase
Formulation 50 mM HEPES, pH 7.4, containing 10% glycerol and 8 µM BH4
Purity cell lysate 100,000 x g supernatant
Stability 6 months
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Background Reading

Knowles, R.G., and Moncada, S. Nitric oxide synthases in mammals. Biochem J 298 249-258 (1994).

Hevel, J.M., and Marletta, M.A. Nitric-oxide synthase assays. Methods Enzymol 233 250-258 (1994).

Lyons, C.R., Orloff, G.J., and Cunningham, J.M. Molecular cloning and functional expression of an inducible nitric oxide synthase from a murine macrophage cell line. J Biol Chem 267 6370-6374 (1992).

Hevel, J.M., White, K.A., and Marletta, M.A. Purification of the inducible murine macrophage nitric oxide synthase. Identification as a flavoprotein. J Biol Chem 266 22789-22791 (1991).

Hébert, C.A., and Baker, J.B. Interleukin-8: A review. Cancer Invest 11 743-750 (1993).

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Size Price Quantity Subtotal
50 units $107.00 $0.00
100 units $203.00 $0.00
250 units $482.00 $0.00
Bulk Contact
Cart Total $0.00

This product is available in custom sizes and/or larger quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

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