iNOS (mouse recombinant)
Cayman Chemical Item Number 60864
NOS II; Inducible Nitric Oxide Synthase
Description
Mr:
130.00 kDa
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Enzyme Commission (EC):
1.14.13.39
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Recombinant enzyme expressed in E. coli · One unit of enzyme produces 1 nmol of NO per minute at 37°C in 50 mM HEPES, pH 7.4, containing 1 mM arginine, 1 mM magnesium acetate, 5 µM oxyhemoglobin, 0.1 mM NADPH, 12 µM tetrahydrobiopterin, and 170 µM DTT.1,2
1
Hevel, J.M., and Marletta, M.A. Nitric-oxide synthase assays. Methods Enzymol 233 250-258 (1994).
2
Lyons, C.R., Orloff, G.J., and Cunningham, J.M. Molecular cloning and functional expression of an inducible nitric oxide synthase from a murine macrophage cell line. J Biol Chem 267 6370-6374 (1992).
| Synonyms |
- NOS II
- Inducible Nitric Oxide Synthase
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| Formulation |
50 mM HEPES, pH 7.4, containing 10% glycerol and 8 µM BH4 |
| Purity |
cell lysate 100,000 x g supernatant |
| Stability |
6 months |
| Storage |
-80°C |
| Shipping |
Dry ice
in continental US; may vary elsewhere
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Background Reading
Knowles, R.G., and Moncada, S. Nitric oxide synthases in mammals. Biochem J 298 249-258 (1994).
Hevel, J.M., and Marletta, M.A. Nitric-oxide synthase assays. Methods Enzymol 233 250-258 (1994).
Lyons, C.R., Orloff, G.J., and Cunningham, J.M. Molecular cloning and functional expression of an inducible nitric oxide synthase from a murine macrophage cell line. J Biol Chem 267 6370-6374 (1992).
Hevel, J.M., White, K.A., and Marletta, M.A. Purification of the inducible murine macrophage nitric oxide synthase. Identification as a flavoprotein. J Biol Chem 266 22789-22791 (1991).
Hébert, C.A., and Baker, J.B. Interleukin-8: A review. Cancer Invest 11 743-750 (1993).
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Pricing updated 2012-02-12.
Prices are subject to change without notice.
To ask for assistance with one of our products please contact a Technical Support Scientist.
Warning This product is not for human or veterinary use.
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