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nNOS (rat recombinant)

Cayman Chemical Item Number 60870

ncNOS; Neuronal Nitric Oxide Synthase; NOS I

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Description

Mr: 150 kDa · Enzyme Commission (EC): 1.14.13.39 · Recombinant enzyme isolated from a Baculovirus overexpression system in Sf9 cells · One unit of enzyme produces 1 nmol of nitric oxide per minute at 37°C in 50 mM HEPES, pH 7.4, containing 5 µM oxyhemoglobin, 50 µM L-arginine, 1 mM CaCl2, 20 µg/ml calmodulin, 120 µM NADPH, 12 µM tetrahydrobiopterin, and 170 µM DTT.1,2

1 Hevel, J.M., and Marletta, M.A. Nitric-oxide synthase assays. Methods Enzymol 233 250-258 (1994).

2 Richards, M.K., and Marletta, M.A. Characterization of neuronal nitric oxide synthase and a C415H mutant, purified from a baculovirus overexpression system. Biochemistry 33 14723-14732 (1994).

Synonyms
  • ncNOS
  • Neuronal Nitric Oxide Synthase
  • NOS I
Formulation A solution in 50 mM HEPES, pH 7.4, containing 10% glycerol and 100 µM DTT
Purity cell lysate 100,000 x g supernatant
Stability 1 year
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Background Reading

Knowles, R.G., and Moncada, S. Nitric oxide synthases in mammals. Biochem J 298 249-258 (1994).

Richards, M.K., and Marletta, M.A. Characterization of neuronal nitric oxide synthase and a C415H mutant, purified from a baculovirus overexpression system. Biochemistry 33 14723-14732 (1994).

Hevel, J.M., and Marletta, M.A. Nitric-oxide synthase assays. Methods Enzymol 233 250-258 (1994).
Size Price Quantity Subtotal
50 units $86.00 $0.00
100 units $163.00 $0.00
250 units $387.00 $0.00
500 units $688.00 $0.00
Bulk Contact
Cart Total $0.00

This product is available in custom sizes and/or larger quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-26. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

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