M_r: 150 kDa · Specific activity: >500 units/mg · Enzyme Commission (EC): 1.14.13.39 · Recombinant enzyme isolated from a Baculovirus overexpression system in Sf9 cells · One unit of enzyme produces 1 nmol of nitric oxide per minute at 37°C in 50 mM HEPES, pH 7.4, containing 5 µM oxyhemoglobin, 1 mM CaCl₂, 20 µg/ml calmodulin, 0.1 mM NADPH, 50 µM arginine, 12 µM tetrahydrobiopterin, and 170 µM DTT.^1,2

1 Hevel, J.M., and Marletta, M.A. Nitric-oxide synthase assays. Methods Enzymol 233 250-258 (1994).

2 Richards, M.K., and Marletta, M.A. Characterization of neuronal nitric oxide synthase and a C415H mutant, purified from a baculovirus overexpression system. Biochemistry 33 14723-14732 (1994).

Knowles, R.G., and Moncada, S. Nitric oxide synthases in mammals. Biochem J 298 249-258 (1994).

Richards, M.K., and Marletta, M.A. Characterization of neuronal nitric oxide synthase and a C415H mutant, purified from a baculovirus overexpression system. Biochemistry 33 14723-14732 (1994).

Abu-Soud, H.M., Feldman, P.L., Clark, P., et al. Electron transfer in the nitric-oxide synthases. Characterization of L-arginine analogs that block heme iron reduction. J Biol Chem 269 32318-32326 (1994).

Hevel, J.M., and Marletta, M.A. Nitric-oxide synthase assays. Methods Enzymol 233 250-258 (1994).

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