M_r: 135 kDa · Enzyme Commission (EC): 1.14.13.39 · Recombinant enzyme isolated from a Baculovirus overexpression system in Sf9 cells · One unit of enzyme produces 1 nmol of NO per minute at 37°C in 50 mM HEPES, pH 7.4, containing 50 µM arginine, 1 mM CaCl₂, 5 µM oxyhemoglobin, 20 µg/ml calmodulin, 0.1 mM NADPH, 12 µM tetrahydrobiopterin, and 170 µM DTT.^1,2,3,4

1 Hevel, J.M., and Marletta, M.A. Nitric-oxide synthase assays. Methods Enzymol 233 250-258 (1994).

2 Busconi, L., and Michel, T. Recombinant endothelial nitric oxide synthase: Post-translational modifications in a baculovirus expression system. Mol Pharmacol 47 655-659 (1995).

3 Sase, K., and Michel, T. Expression and regulation of endothelial nitric oxide synthase. Trends Cardiovasc Med 7 28-37 (1997).

4 Verhaar, M.C., Wever, R.M.F., Kastelein, J.J.P., et al. 5-Methyltetrahydrofolate, the active form of folic acid, restores endothelial function in familial hypercholesterolemia. Circulation 97 237-241 (1998).

Knowles, R.G., and Moncada, S. Nitric oxide synthases in mammals. Biochem J 298 249-258 (1994).

Kobzar, G., Shelkovnikov, S., Mardla, V., et al. A 15-nonstereogenic carbocyclic analogue of prostacyclin: Effects on human platelets and uterine artery. J Lipid Mediat Cell Signal 10 2443-2449 (1994).

Garvey, E.P., Oplinger, J.A., Tanoury, G.J., et al. Potent and selective inhibition of human nitric oxide synthases. Inhibition by non-amino acid isothioureas. J Biol Chem 269 26669-26676 (1994).

Lee, C.M., Robinson, L.J., and Michel, T. Oligomerization of endothelial nitric oxide synthase. Evidence for a dominant negative effect of truncation mutants. J Biol Chem 270 27403-27406 (1995).

Verhaar, M.C., Wever, R.M.F., Kastelein, J.J.P., et al. 5-Methyltetrahydrofolate, the active form of folic acid, restores endothelial function in familial hypercholesterolemia. Circulation 97 237-241 (1998).

Sase, K., and Michel, T. Expression and regulation of endothelial nitric oxide synthase. Trends Cardiovasc Med 7 28-37 (1997).

Busconi, L., and Michel, T. Recombinant endothelial nitric oxide synthase: Post-translational modifications in a baculovirus expression system. Mol Pharmacol 47 655-659 (1995).

Hevel, J.M., and Marletta, M.A. Nitric-oxide synthase assays. Methods Enzymol 233 250-258 (1994).

Shaul, P.W., North, A.J., Wu, L.C., et al. Endothelial nitric oxide synthase is expressed in cultured human bronchiolar epithelium. J Clin Invest 94 2231-2236 (1994).

Show all 9 Hide all but first 3