See personalized New Product recommendations! Get personalized New Product recommendations! Register or Login for personalized New Product recommendations!

Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

Myeloperoxidase Peroxidation Assay Kit

Cayman Chemical Item Number 700160

MPO Peroxidation Assay Kit

Myeloperoxidase Peroxidation Assay Kit

See more

Description

Myeloperoxidase (MPO) is a member of the heme peroxidase superfamily and is stored within the azurophilic granules of leukocytes.1 MPO is found within circulating neutrophils, monocytes, and some tissue macrophages.2 A unique activity of MPO is its ability to use chloride as a cosubstrate with hydrogen peroxide to generate chlorinating oxidants such as hypochlorous acid, a potent antimicrobial agent.3 Recently, evidence has emerged that MPO-derived oxidants contribute to tissue damage and the initiation and propagation of acute and chronic vascular inflammatory diseases.4,5 The fact that circulating levels of MPO have been shown to predict risks for major adverse cardiac events and that levels of MPO-derived chlorinated compounds are specific biomarkers for disease progression, has attracted considerable interest in the development of therapeutically useful MPO inhibitors.6 MPO also oxidizes a variety of substrates, including phenols and anilines, via the classic peroxidation cycle. The relative concentrations of chloride and the reducing substrate determine whether MPO uses hydrogen peroxide for chlorination or peroxidation. Cayman’s MPO Peroxidation Assay provides a convenient fluorescence-based method for detecting the MPO peroxidase activity in both crude cell lysates and purified enzyme preparations. The assay utilizes the peroxidase component of MPO. The reaction between hydrogen peroxide and ADHP (10-acetyl-3,7-dihydroxyphenoxazine) produces the highly fluorescent compound resorufin. Resorufin fluorescence can be easily analyzed with an excitation wavelength of 530-540 nm and emission wavelength of 585-595 nm. The kit includes a MPO-specific inhibitor for distinguishing between MPO activity from MPO-independent fluorescence.

1 Yamada, M., and Kurahashi, K. Regulation of myeloperoxidase gene expression during differentiation of human myeloid leukemia HL-60 cells. J Biol Chem 259(5) 3021-3025 (1984).

2 Schultz, J., and Kaminker, K. Myeloperoxidase of the leucocyte of normal human blood.1 I. Content and localization. Arch Biochem Biophys 96 465-467 (1962).

3 Harrison, J.E., and Schultz, J. Studies on the chlorinating activity of myeloperoxidase. J Biol Chem 251(5) 1371-1374 (1976).

4 Podrez, E.A., Abu-Soud, H.M., and Hazen, S.L. Myeloperoxidase-generated oxidants and atherosclerosis. Free Radic Biol Med 28(12) 1717-1725 (2000).

5 Zhang, R., Brennan, M., Fu, X., et al. Association between myeloperoxidase levels and risk of coronary artery disease. JAMA 286(17) 2136-2142 (2001).

6 Malle, E., Furtmüller, P.G., Sattler, W., et al. Myeloperoxidase: A target for new drug development? Br J Pharmacol 152 838-854 (2007).

Synonyms
  • MPO Peroxidation Assay Kit
Stability 6 months
Storage 4°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Yamada, M., and Kurahashi, K. Regulation of myeloperoxidase gene expression during differentiation of human myeloid leukemia HL-60 cells. J Biol Chem 259(5) 3021-3025 (1984).

Kettle, A.J., Gedye, C.A., and Winterbourn, C.C. Mechanism of inactivation of myeloperoxidase by 4-aminobenzoic acid hydrazide. Biochem J 321 503-508 (1997).

Podrez, E.A., Abu-Soud, H.M., and Hazen, S.L. Myeloperoxidase-generated oxidants and atherosclerosis. Free Radic Biol Med 28(12) 1717-1725 (2000).

Zhang, R., Brennan, M., Fu, X., et al. Association between myeloperoxidase levels and risk of coronary artery disease. JAMA 286(17) 2136-2142 (2001).

Malle, E., Furtmüller, P.G., Sattler, W., et al. Myeloperoxidase: A target for new drug development? Br J Pharmacol 152 838-854 (2007).

Kettle, A.J., Gedye, C.A., Hampton, M.B., et al. Inhibition of myeloperoxidase by benzoic acid hydrazides. Biochem J 308 559-563 (1995).

Schultz, J., and Kaminker, K. Myeloperoxidase of the leucocyte of normal human blood.1 I. Content and localization. Arch Biochem Biophys 96 465-467 (1962).

Harrison, J.E., and Schultz, J. Studies on the chlorinating activity of myeloperoxidase. J Biol Chem 251(5) 1371-1374 (1976).

Show all 8 Hide all but first 3
700160-192well
96-Well Cover Sheet
96-Well Solid Plate (black)
MPO Peroxidation Substrate 5 × 1 ea
MPO Resorufin Standard 1 ea
MPO DMSO 1 ea
MPO Assay Buffer 1 ea
Myeloperoxidase Control 1 ea
MPO Inhibitor 1 ea
MPO Hydrogen Peroxide 1 ea
Size Price Quantity Subtotal
2 x 96 wells $219.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Myeloperoxidase Peroxidation Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

Related Products

10-Acetyl-3,7-dihydroxyphenoxazine
Catalase Assay Kit
Myeloperoxidase Chlorination Assay Kit
Myeloperoxidase (human) EIA Kit
Myeloperoxidase Inhibitor Screening Assay Kit
Show all 5 Hide all but first 3

Downloads

Batch-specific Information

Login to access batch-specific information

Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

Other Resources
Management Team Company Profile Company History ChemAssistant Tools FAQs Cayman Europe Cayman Pharma CaBRI Cayman Gear Press Releases Illustrations and Charts Key Research Area Posters Article Library Analysis Tools Conference Schedule Order Terms Browser Recommendation Privacy Statement Site Map

Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA

Toll Free: (800) 364-9897 (USA and Canada Only) · Fax: (734) 971-3640

Copyright 2012 Cayman Chemical Company

Lost password?