HDAC8 Inhibitor Screening Assay Kit

Cayman Chemical Item Number 700230

Histone Deacetylase 8 Inhibitor Screening Assay Kit

Description

Human HDAC8 is a class I HDAC and has been identified in a variety of human cancer tissues. Cayman’s HDAC8 Inhibitor Screening Assay provides a convenient fluorescence-based method for screening HDAC8 inhibitors. The procedure requires only two easy steps, both performed in the same microplate. The fluorescent reaction product is analyzed with an excitation wavelength between 350-360 nm and an emission wavelength between 450-465 nm. Sufficient HDAC8 is provided for 100 tests.

Synonyms

Histone Deacetylase 8 Inhibitor Screening Assay Kit

Stability

1 year

Storage

-80°C

Shipping

Dry ice in continental US; may vary elsewhere

Background Reading

Pan, L., Lu, J., and Huang, B. HDAC inhibitors: A potential new category of anti-tumor agents. Cell Mol Immunol 4(5) 337-343 (2007).

Yang, X., and Seto, E. The Rpd3/Hda1 family of lysine deacetylases: From bacteria and yeast to mice and men. Nat Rev Mol Cell Biol 9(3) 206-213 (2008).

Oehme, I., Deubzer, H.E., Wegener, D., et al. Histone deacetylase 8 in neuroblastoma tumorigenesis. Clin Cancer Res 15(1) 91-99 (2009).

Nakagawa, M., Oda, Y., Eguchi, T., et al. Expression profile of class I histone deacetylases in human cancer tissues. Oncol Rep 18 769-774 (2007).

Grunstein, M. Histone acetylation in chromatin structure and transcription. Nature 389 349-352 (1997 Sep 25).

Strahl, B.D., and Allis, D. The language of covalent histone modifications. Nature 403 41-45 (2000 Jan 6).

Cheung, W.L., Briggs, D.B., and Allis, C.D. Acetylation and chromosomal functions. Curr Opin Cell Biol 12 326-333 (2000 Jan 1).

Kwon, H.J., Kim, M.S., Kim, M.J., et al. Histone deacetylase inhibitor FK228 inhibits tumor angiogenesis. Int J Cancer 97 290-296 (2002 Jan 1).

Suenaga, M., Soda, H., Oka, M., et al. Histone deacetylase inhibitors suppress telomerase reverse transcriptase mRNA expression in prostate cancer cells. Int J Cancer 97 621-625 (2002 Jan 1).

Johnstone, R.W. Histone-deacetylase inhibitors: Novel drugs for the treatment of cancer. Nat Rev Drug Discov 1 287-299 (2002).

Hu, E., Chen, Z., Fredrickson, T., et al. Cloning and characterization of a novel human class I histone deacetylase that functions as a transcription repressor. J Biol Chem 275(20) 15254-15264 (2000).

Buggy, J.J., Sideris, M.L., Mak, P., et al. Cloning and characterization of a novel human histone deacetylase, HDAC8. Biochem J 350 199-205 (2000).

Waltregny, D., Glénisson, W., Tran, S.L., et al. Histone deacetylase HDAC8 associates with smooth muscle a-actin and is essential for smooth muscle cell contractility. FASEB J 19(8) 966-968 (2005).

Show all 13 Hide all but first 3

700230-96well

Half Volume 96-Well Solid Plate (white)