Phosphatidic acid (PA) is a common phospholipid that is a major constituent of cell membranes and a central intermediate for the synthesis of membrane lipids and storage lipids. Cayman’s Phosphatidic Acid Assay provides a fluorescence-based method for measuring PA. Lipase is used to hydrolyze PA in cellular lipids to glycerol-3-phosphate. A coupled enzyme reaction system generates the highly fluorescent compound resorufin (excitation 530-540 nm and emission 585-595 nm).

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